Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/5938
Título: Identification of sites of injury in Lactobacillus bulgaricus during heat stress
Autor: Teixeira, P.
Castro, H.
Mohácsi-Farkas, C.
Kirby, R.
Data: 1997
Editora: Wiley Blackwell
Citação: TEIXEIRA, P... [et al.] - Identification of sites of injury in Lactobacillus bulgaricus during heat stress. Journal of Applied Microbiology. ISSN 1365-2672. Vol. 83, n.º 2 (1997), p. 219–226
Resumo: Heat resistance of Lactobacillus bulgaricus in skimmed milk at 62°, 64°, 65° and 66°C was studied. The response to increasing temperatures in this range was not linear, with temperatures at 65°C and above giving a lower survival rate than would be predicted from experiments at lower temperatures. To identify sites of injury at these temperatures, chemical markers were used. Heating at 64°C and below resulted in damage to the cytoplasmic membrane. At temperatures of 65°C and above chemical markers also indicated damage in the cell wall and proteins. Using differential scanning calorimetry analysis of whole cells of Lact. bulgaricus seven main peaks were observed (l–51, m1–61, m2–73, n–80, p–89, q–100,r–112°C). Three of these peaks (lr, mr and pr) were the result of reversible reactions. Analysis of cell fractions identified the cell structure involved in giving rise to each of the three reversible peaks; lr, cell membrane lipids, mr, ribosomes, and pr, DNA. The evidence presented in this paper shows that irreversible reactions in the cell ribosomes are a critical site of damage in Lact. bulgaricus during heat stress in liquid media at 65°C and above.
Peer review: yes
URI: http://hdl.handle.net/10400.14/5938
Versão do Editor: The definitive version is available at http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2672.1997.00221.x/abstract
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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