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Identification of sites of injury in Lactobacillus bulgaricus during heat stress

1997Journal article Open access
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dc.contributor.authorTeixeira, P.
dc.contributor.authorCastro, H.
dc.contributor.authorMohácsi-Farkas, C.
dc.contributor.authorKirby, R.
dc.date.accessioned2011-10-11T11:10:30Z
dc.date.available2011-10-11T11:10:30Z
dc.date.issued1997
dc.description.abstractHeat resistance of Lactobacillus bulgaricus in skimmed milk at 62°, 64°, 65° and 66°C was studied. The response to increasing temperatures in this range was not linear, with temperatures at 65°C and above giving a lower survival rate than would be predicted from experiments at lower temperatures. To identify sites of injury at these temperatures, chemical markers were used. Heating at 64°C and below resulted in damage to the cytoplasmic membrane. At temperatures of 65°C and above chemical markers also indicated damage in the cell wall and proteins. Using differential scanning calorimetry analysis of whole cells of Lact. bulgaricus seven main peaks were observed (l–51, m1–61, m2–73, n–80, p–89, q–100,r–112°C). Three of these peaks (lr, mr and pr) were the result of reversible reactions. Analysis of cell fractions identified the cell structure involved in giving rise to each of the three reversible peaks; lr, cell membrane lipids, mr, ribosomes, and pr, DNA. The evidence presented in this paper shows that irreversible reactions in the cell ribosomes are a critical site of damage in Lact. bulgaricus during heat stress in liquid media at 65°C and above.por
dc.identifier.citationTEIXEIRA, P... [et al.] - Identification of sites of injury in Lactobacillus bulgaricus during heat stress. Journal of Applied Microbiology. ISSN 1365-2672. Vol. 83, n.º 2 (1997), p. 219–226por
dc.identifier.doi10.1046/j.1365-2672.1997.00221.x
dc.identifier.urihttp://hdl.handle.net/10400.14/5938
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWiley Blackwellpor
dc.titleIdentification of sites of injury in Lactobacillus bulgaricus during heat stresspor
dc.typejournal article
dspace.entity.typePublication
person.familyNameTeixeira
person.givenNamePaula
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id7005895206
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb918d4a2-b9f9-4c56-8318-111625e4d58b
relation.isAuthorOfPublication.latestForDiscoveryb918d4a2-b9f9-4c56-8318-111625e4d58b

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