Name: | Description: | Size: | Format: | |
---|---|---|---|---|
130.82 KB | Adobe PDF |
Advisor(s)
Abstract(s)
A detailed classification of a novel bacterial strain, designated F11T, capable of degrading fluorobenzene as a sole carbon and energy source, was performed by using a polyphasic approach. This Gram-negative, rod-shaped, non-motile, non-spore-forming, aerobic bacterium was isolated from a sediment sample collected from an industrially contaminated site in northern
Portugal. The predominant whole-cell fatty acids were C19 : 0 cyclo ω8c, C16 : 01 C18 : 1ω7c, C18 : 0,C18 : 0 3-OH and C16 : 0 3-OH. The G+C content of the DNA was 62.9 mol% and the major respiratory quinone was ubiquinone 10 (UQ-10). 16S rRNA gene sequence analysis revealed that strain F11T was a member of the class Alphaproteobacteria and was phylogenetically related to the genus Labrys, having sequence similarities of 95.6 and 93.1% to the type strains of Labrys
monachus and Labrys methylaminiphilus, respectively. DNA–DNA hybridization experiments revealed levels of relatedness of ,70% between strain F11T and the type strains of L. monachus and L. methylaminiphilus (38.6 and 34.1 %, respectively), justifying the classification of strain F11T as representing a novel species of the genus Labrys. The name Labrys portucalensis sp. nov. is proposed for this organism. The type strain is F11T (5LMG 23412T5DSM 17916T).
Description
Keywords
Pedagogical Context
Citation
"International Journal of Systematic and Evolutionary Microbiology". ISSN 1466-5026. 58: 3 (2008) 692–698
Publisher
Society for General Microbiology