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Advisor(s)
Abstract(s)
A sequential injection lab-on valve (SI-LOV) method for the enzymatic determination of hydrogen peroxide
was developed. The spectrophotometric assay is based on the reaction between hydrogen peroxide and ABTS
(2,2′-Azino-bis(3-Ethylbenzothiazoline 6-sulfonic acid)) in the presence of the enzyme HRP (horseradish
peroxidase). The produced oxidized ABTS is measured at 410 nm. The sample consumption was 15 μL/assay
and the consumption of HRP and ABTS was 34.6 mg L−1 and 0.06 g L−1, respectively with a determination rate
of 45 h−1.
Relative deviations lower than 9.0% were found when the results were compared to those obtained by the
reference procedure in the analysis of bleaches and disinfection solutions for contact lenses. With the
incorporation of an in-line dilution (dialysis) process,was possible to attain a response range up to 342 mg L−1
of hydrogen peroxide. The developed methodwas applied to monitor on-line of the disinfection–neutralization
process of contact lenses. The study of two different one-step systems for cleaning contact lenses demonstrated
that the neutralization of the hydrogen peroxide is completed within 6 h as recommended by the
manufactures. The developed flow method was proved to be a useful tool for monitoring the dynamic process
of disinfection–neutralization.
Description
Keywords
SI-LOV Hydrogen peroxide Horseradish peroxidase Contact lenses
Pedagogical Context
Citation
"Microchemical Journal". ISSN 0026-265X. 91: 2 (2009) 197–201
Publisher
Elsevier