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Abstract(s)
Listeria monocytogenes infection (listeriosis) is an uncommon but severe foodborne illness that affects mainly
individuals with recognized underlying conditions: the elderly, immunocompromised individuals, and pregnant
women and their fetuses. The aim of this study was to obtain epidemiological data on cases of listeriosis
occurring in Portugal from 2008 through 2012, collected in hospitals on a voluntary basis. L. monocytogenes
isolates were characterized by genoserotyping by multiplex polymerase chain reaction, DNA macrorestriction
pulsed-field gel electrophoresis (PFGE), and determination of minimal inhibitory concentration (MIC, lg/mL)
for 12 antibiotics. During this period, 203 cases of listeriosis were detected. The annual incidence rate observed
ranged from 0.2 to 0.7 cases per 100,000 inhabitants. Nineteen cases (9.5%) corresponded to maternal/neonatal
(MN) infections. The mean age of the nonmaternal/neonatal (non-MN) cases with documented age was 59
years, and 46.4% occurred in patients aged over 65 years. The majority of listeriosis cases were caused by
genoserogroup IVb isolates, and PFGE analysis revealed a high molecular diversity, suggesting that most were
sporadic. Nevertheless, several clusters of isolates presenting different geographic and time distributions were
detected. The incidence of antibiotic-resistant isolates of L. monocytogenes was low but significantly higher
than in previous years (2003–2007). The implementation of a national surveillance system monitoring the
incidence of listeriosis and antimicrobial resistance of strains would be most valuable, allowing identification of
sporadic and outbreak cases, to detect general trends in antibiotic susceptibilities, and potentially identify food
sources of clinical strains.
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Pedagogical Context
Citation
MAGALHÃES, Rui …[et al.] - Genetic and phenotypic characterization of Listeria monocytogenes from human clinical cases occurred in Portugal between 2008 and 2012. Foodborne Pathogens and Disease. ISSN 1556-7125. Vol. 11, n.º 11 (2014), p. 1-10
Publisher
Mary Ann Liebert