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Genetic and phenotypic characterization of Listeria monocytogenes from human clinical cases occurred in Portugal between 2008 and 2012

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dc.contributor.authorMagalhães, Rui
dc.contributor.authorFerreira, Vânia
dc.contributor.authorSantos, Isabel
dc.contributor.authorAlmeida, Gonçalo
dc.contributor.authorTeixeira, Paula
dc.contributor.authorResearch Team
dc.date.accessioned2015-05-15T10:59:19Z
dc.date.available2015-05-15T10:59:19Z
dc.date.issued2014
dc.description.abstractListeria monocytogenes infection (listeriosis) is an uncommon but severe foodborne illness that affects mainly individuals with recognized underlying conditions: the elderly, immunocompromised individuals, and pregnant women and their fetuses. The aim of this study was to obtain epidemiological data on cases of listeriosis occurring in Portugal from 2008 through 2012, collected in hospitals on a voluntary basis. L. monocytogenes isolates were characterized by genoserotyping by multiplex polymerase chain reaction, DNA macrorestriction pulsed-field gel electrophoresis (PFGE), and determination of minimal inhibitory concentration (MIC, lg/mL) for 12 antibiotics. During this period, 203 cases of listeriosis were detected. The annual incidence rate observed ranged from 0.2 to 0.7 cases per 100,000 inhabitants. Nineteen cases (9.5%) corresponded to maternal/neonatal (MN) infections. The mean age of the nonmaternal/neonatal (non-MN) cases with documented age was 59 years, and 46.4% occurred in patients aged over 65 years. The majority of listeriosis cases were caused by genoserogroup IVb isolates, and PFGE analysis revealed a high molecular diversity, suggesting that most were sporadic. Nevertheless, several clusters of isolates presenting different geographic and time distributions were detected. The incidence of antibiotic-resistant isolates of L. monocytogenes was low but significantly higher than in previous years (2003–2007). The implementation of a national surveillance system monitoring the incidence of listeriosis and antimicrobial resistance of strains would be most valuable, allowing identification of sporadic and outbreak cases, to detect general trends in antibiotic susceptibilities, and potentially identify food sources of clinical strains.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationMAGALHÃES, Rui …[et al.] - Genetic and phenotypic characterization of Listeria monocytogenes from human clinical cases occurred in Portugal between 2008 and 2012. Foodborne Pathogens and Disease. ISSN 1556-7125. Vol. 11, n.º 11 (2014), p. 1-10por
dc.identifier.doi10.1089/fpd.2014.1806
dc.identifier.issn1535-3141
dc.identifier.urihttp://hdl.handle.net/10400.14/17612
dc.identifier.wosWOS:000344668600011
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherMary Ann Liebert
dc.relationNORTE-07-0124-FEDER-000052
dc.relationCHARACTERISTICS OF SPECIFIC STRAINS OF LISTERIA MONOCYTOGENES POTENTIATING PERSISTENCE IN FOOD AND FOOD PROCESSING ENVIRONMENTS AND RELEVANCE TO FOOD SAFETY
dc.titleGenetic and phenotypic characterization of Listeria monocytogenes from human clinical cases occurred in Portugal between 2008 and 2012por
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCHARACTERISTICS OF SPECIFIC STRAINS OF LISTERIA MONOCYTOGENES POTENTIATING PERSISTENCE IN FOOD AND FOOD PROCESSING ENVIRONMENTS AND RELEVANCE TO FOOD SAFETY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F71704%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F72617%2F2010/PT
oaire.citation.endPage916
oaire.citation.issue11
oaire.citation.startPage907
oaire.citation.titleFoodborne Pathogens and Disease
oaire.citation.volume11
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameMagalhães
person.familyNameBorges Ferreira
person.familyNameAlmeida
person.familyNameTeixeira
person.givenNameRui
person.givenNameVânia
person.givenNameGonçalo
person.givenNamePaula
person.identifierK-1359-2014
person.identifier267767
person.identifier.ciencia-id4C1A-BDD4-D716
person.identifier.ciencia-id341E-5F76-548C
person.identifier.orcid0000-0003-3172-440X
person.identifier.orcid0000-0001-7093-9193
person.identifier.orcid0000-0003-1513-0951
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridK-1359-2014
person.identifier.ridK-1343-2014
person.identifier.ridK-4389-2014
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id6603813414
person.identifier.scopus-author-id8317338900
person.identifier.scopus-author-id7003759404
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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