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Abstract(s)
A group of biofilm-producing bacteria isolated from patients with urinary tract infections was
evaluated, identifying the main factors contributing to biofilm formation. Among the 156 isolates,
58 (37.2 %) were biofilm producers. The bacterial species (P,0.001), together with patient’s
gender (P50.022), were the factors with the highest influence for biofilm production. There was
also a strong correlation of catheterization with biofilm formation, despite being less significant
(P50.070) than species or gender. In fact, some of the bacteria isolated were biofilm producers in
all cases. With regard to resistance profile among bacterial isolates, b-lactam antibiotics
presented the highest number of cases/percentages – ampicillin (32/55.2 %), cephalothin (30/
51.7 %), amoxicillin/clavulanic acid (22/37.9 %) – although the carbapenem group still
represented a good therapeutic option (2/3.4 %). Quinolones (nucleic acid synthesis inhibitors)
also showed high resistance percentages. Furthermore, biofilm production clearly increases
bacterial resistance. Almost half of the biofilm-producing bacteria showed resistance against at
least three different groups of antibiotics. Bacterial resistance is often associated with
catheterization. Accordingly, intrinsic (age and gender) and extrinsic (hospital unit, bacterial isolate
and catheterization) factors were used to build a predictive model, by evaluating the contribution
of each factor to biofilm production. In this way, it is possible to anticipate biofilm occurrence
immediately after bacterial identification, allowing selection of a more effective antibiotic (among
the susceptibility options suggested by the antibiogram) against biofilm-producing bacteria. This
approach reduces the putative bacterial resistance during treatment, and the consequent need to
adjust antibiotherapy
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Pedagogical Context
Citation
ALVES, Maria José …[et al.] - Propensity for biofilm formation by clinical isolates from urinary tract infections: Developing a multifactorial predictive model to improve antibiotherapy. Journal of Medical Microbiology. ISSN 0022-2615. N.º 63, Part.3 (2014), p. 471-477
Publisher
Microbiology Society