Publicação
An SNF2 helicase-like protein links mitotic transcription termination to sister chromatid resolution
| dc.contributor.author | Carmo, Catarina | |
| dc.contributor.author | Coelho, João | |
| dc.contributor.author | Silva, Rui | |
| dc.contributor.author | Tavares, Alexandra | |
| dc.contributor.author | Boavida, Ana | |
| dc.contributor.author | Gaetani, Paola | |
| dc.contributor.author | Martinho, Rui Gonçalo | |
| dc.contributor.author | Oliveira, Raquel A. | |
| dc.date.accessioned | 2022-11-30T13:31:16Z | |
| dc.date.available | 2022-11-30T13:31:16Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Mitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs after substantial chromosome compaction and is not promoted by condensin I. Instead, we show that the timely removal of transcripts from mitotic chromatin is driven by the SNF2 helicase-like protein Lodestar (Lds), identified here as a modulator of sister chromatid cohesion defects. In addition to transcriptional termination, we uncovered that Lds cooperates with Topoisomerase 2 to ensure efficient sister chromatid resolution and mitotic fidelity. We conclude that mitotic transcriptional termination is not a passive consequence of cell cycle progression and/or chromosome compaction but occurs via dedicated mechanisms with functional parallelisms to sister chromatid resolution. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.1101/2022.11.21.517340 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.14/39449 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | no | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.title | An SNF2 helicase-like protein links mitotic transcription termination to sister chromatid resolution | pt_PT |
| dc.type | preprint | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | preprint | pt_PT |