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Changes in red blood cells membrane protein composition during hemodialysis procedure

2008-11Journal article Open access
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dc.contributor.authorCosta, Elísio
dc.contributor.authorRocha, Susana
dc.contributor.authorRocha-Pereira, Petronila
dc.contributor.authorCastro, Elisabeth
dc.contributor.authorMiranda, Vasco
dc.contributor.authorFaria, Maria do Sameiro
dc.contributor.authorLoureio, Alfredo
dc.contributor.authorQuintanilha, Alexandre
dc.contributor.authorBelo, Luís
dc.contributor.authorSantos-Silva, Alice
dc.date.accessioned2010-12-13T10:10:12Z
dc.date.available2010-12-13T10:10:12Z
dc.date.issued2008-11
dc.description.abstractOur aim was to evaluate the influence of the hemodialysis (HD) procedure in red blood cells (RBC) membrane protein composition. We evaluated hematological data (RBC count, hemoglobin concentration, and hematimetric indices) and RBC membrane protein composition (linear and exponential gradient polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate [SDS-PAGE] followed by densitometry analysis of RBC membrane proteins) before and immediately after the HD procedure in 20 patients (10 responders and 10 non-responders to recombinant human erythropoietin therapy [rhEPO]) and 26 healthy controls. Before HD, patients presented anaemia and significant changes in membrane protein composition, namely, a statistically significant reduction in spectrin associated with a significant increase in bands 6, as well as an altered membrane protein interaction (protein 4.1/spectrin, protein 4.1/band 3, protein 4.2/band 3 and spectrin/band 3). After HD, we found that patients showed a statistically significant increase in RBC count and hemoglobin, a further and statistically significant decrease in spectrin, an increase in band 3, and an altered spectrin/band 3 ratio. When comparing responders and non-responders patients after HD, we found that the non-responders presented a trend to a higher reduction in spectrin. Our data suggest that HD procedure seems to contribute to a reduction in spectrin, which is normally associated with a reduction in RBC deformability, being that reduction in spectrin is higher in non-responder patients.por
dc.identifier.citation"Renal Failure" . ISSN 0886-022X. 30:10 (2008) 971–975por
dc.identifier.doi10.1080/08860220802422036
dc.identifier.urihttp://hdl.handle.net/10400.14/3796
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherInforma Healthcarepor
dc.subjectChronic kidney diseasepor
dc.subjectChronic renal failurepor
dc.subjectHemodialysispor
dc.subjectSpectrinpor
dc.subjectErythrocyte membranepor
dc.subjectResistance to rhEPOpor
dc.titleChanges in red blood cells membrane protein composition during hemodialysis procedurepor
dc.typejournal article
dspace.entity.typePublication
person.familyNameBelo
person.givenNameLuís
person.identifier.ciencia-idC319-EED1-E15D
person.identifier.orcid0000-0002-3941-6850
person.identifier.ridK-5878-2013
person.identifier.scopus-author-id6602879850
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication3654b7b5-7ad2-4f2f-9114-93dc094f8e9e
relation.isAuthorOfPublication.latestForDiscovery3654b7b5-7ad2-4f2f-9114-93dc094f8e9e

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