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Chiral HPLC-FD method validation for determination of several Beta-Blockers and Fluoxetine in biodegradation assays

dc.contributor.authorRibeiro, Ana R.
dc.contributor.authorCastro, Paula M.L.
dc.contributor.authorAfonso, Carlos
dc.contributor.authorTiritan, Maria E.
dc.date.accessioned2013-11-14T16:20:17Z
dc.date.available2013-11-14T16:20:17Z
dc.date.issued2011
dc.description.abstractChiral pharmaceuticals and the fate and effects of their enantiomers in the environment are still largely unknown [1, 2]. Enantiomers have different interactions with enzymes, receptors and any chiral molecules leading to different biological activities and affecting organisms in a different manner. Thus, biodegradation tends to be enantioselective in contrast to abiotic degradation. The methods developed to quantify the enantiomeric fraction in the environment and to follow biodegradation are scarce [3]. Thus in this work we describe the development and validation of HPLC methods that allow the enantiomeric separation of widely used drugs namely four beta-blockers: alprenolol (ALP), propranolol (PHO), metoprolol (MET) and atenolol (ATE) and the antidepressant fluoxetine (FX). The macrocyclic antibiotic vancomycin CSP (ASTEC Chirobiotic V 5µm) was used under polar organic mode (methanol:ethanol:triethylamine:acetic acid.50:50 v/v) and fluorescence detection for enantiomeric fraction quantification. The developed methods were established using a minimal medium inoculated with activated sludge as a matrix which is the condition used in the biodegradation studies. The vancomycin CSP was able to resolve ALP and PHO as well as MET, ATE and FX in two chromatographic runs. The chromatographic parameters obtained have shown the separation factor (α) between 1.12 and 1.34 and resolution (Rs) between 1.30 and 4.35. The methods demonstrated to be selective and linearity with r2 higher than 0,999 for the range selected. The method detection limits were between 2.5 to 10ng/mL. These methods were applied to follow the biodegradation of the target chiral compounds during 15 days. The biodegradation assays were performed using activated sludge from a WWTP and the results indicate the higher degradation extents for the S- enantiomer forms.por
dc.identifier.citationRIBEIRO, Ana R. [et al.] - Chiral HPLC-FD method validation for determination of several Beta-Blockers and Fluoxetine in biodegradation assays. In 36th International Symposium on High-Performance Liquid Phase Separations and Related Techniques (HPLC 2011), Budapest, Hungary, 19-23 June 2011por
dc.identifier.urihttp://hdl.handle.net/10400.14/13239
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectbiodegradationpor
dc.subjectchiralpor
dc.subjectpharmaceuticalspor
dc.titleChiral HPLC-FD method validation for determination of several Beta-Blockers and Fluoxetine in biodegradation assayspor
dc.typeconference object
dspace.entity.typePublication
person.familyNameCastro
person.familyNameAfonso
person.familyNameTiritan
person.givenNamePaula
person.givenNameCarlos
person.givenNameMaria Elizabeth
person.identifier2013444
person.identifier1310409
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.ciencia-id6A10-3565-6158
person.identifier.ciencia-idD915-BFC0-FB3A
person.identifier.orcid0000-0001-8841-6606
person.identifier.orcid0000-0002-5731-5789
person.identifier.orcid0000-0003-3320-730X
person.identifier.ridM-8241-2013
person.identifier.ridI-5989-2015
person.identifier.ridK-9492-2014
person.identifier.scopus-author-id7102781782
person.identifier.scopus-author-id36145043600
person.identifier.scopus-author-id6602890981
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublicationf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isAuthorOfPublication181a86a6-86a0-463c-b86e-d4e5669236da
relation.isAuthorOfPublication1ab8e8f5-cf82-4edd-89d8-46e5cca13e19
relation.isAuthorOfPublication.latestForDiscovery181a86a6-86a0-463c-b86e-d4e5669236da

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