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Advisor(s)
Abstract(s)
Two methods based on headspace solid-phase
microextraction (HS-SPME) coupled to gas chromatography–
ion trap mass spectrometry (GC-IT/MS) were
proposed for the analysis of volatile organic compounds
(VOCs) in cork material used in the production of cork
stoppers. The effect of various factors affecting the extraction
efficiency was carried out by means of a 24 full factorial
design. The first method allowed the extraction of
17 terpenes by using a divinylbenzene/carboxen/polydimethylsiloxane
(DVB/CAR/PDMS) fiber (50/30 μm). The
optimal conditions were achieved when cork extract (5 mL)
added with 2.3 g of NaCl was extracted during 35 min at
55 °C. The second method allowed the identification of 41
carbonyl compounds after in-solution (5 mL) derivatization
with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride
(PFBHA, 2.3 mg/mL), followed by an incubation
period of 6 min at 52 °C and extraction during 36 min at
the same temperature, using a PDMS/DVB (50/30 μm)
fiber. Both methods are simple, solvent free and fast. These
methods were applied to the analysis of different cork raw material showing significant differences in the amounts
of volatile compounds analyzed. Alcanfor and α-terpineol
were the terpenes compounds present at highest amounts,
and within carbonyl compounds analyzed, some samples
presented a high level of butanal, octanal, nonanal, and
glyoxal.
Description
Keywords
Quercus suber L. Cork Volatile composition HS-SPME–GC-IT/MS Terpenes Carbonyl compounds
Citation
MOREIRA, Nathalie; LOPES, P.; CABRAL, M.; PINHO, P. Guedes de - HS-SPME/GC-MS methodologies for the analysis of volatile compounds in cork material. European Food Research and Technology. ISSN 1438-2385. Vol. 242, n.º 4 (2016), p. 457-466
Publisher
Springer