| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 600.52 KB | Adobe PDF |
Advisor(s)
Abstract(s)
A flow injection analysis system with a spectrophotometric detection was arranged in order to develop a simple
and fast methodology for the quantification of titratable acidity in different types of wines. In the proposed system, a precise volume of sample is injected in the carrier stream, composed by a mixture of OH− solution with the pH indicator, bromothymol blue (BTB). Consequently, a change on the colour of the carrier solution is achieved and quantified by spectrophotometry. Therefore, it was possible to establish a linear range up to 0.16 g/L expressed as tartaric acid, with a limit of detection and quantification of 0.01 and 0.05 g/L, respectively. The proposed work has a low sample and reagents consumption, only 96 μg of BTB, 336 mmol de OH− and 50 μL
of sample were needed per assay. At the same time, a high determination rate was achieved, 75 determinations per hour. The developed methodology was applied on the quantification of total acidity in red and white table wine and port wine samples.
Description
Keywords
Bromothymol blue Flow-injection system Spectrophotometry Titratable acidity Table and port wine
Pedagogical Context
Citation
VIDIGAL, SUSANA S. M. P.; RAMOS, Ana T. C.; RANGEL, António O. S. S. - Flow-Based System for the Determination of Titratable Acidity in Wines. Food Analytical Methods. (2016) ISSN: 1936-9751. Vol. 9, nº 8, p.2241–2245
Publisher
Springer