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Genetic, phenotypic and functional variation within a Glomus geosporum isolate cultivated with or without the stress of a highly alkaline anthropogenic sediment

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dc.contributor.authorOliveira, Rui S.
dc.contributor.authorBoyer, Louisa Robinson
dc.contributor.authorCarvalho, Maria F.
dc.contributor.authorJeffries, Peter
dc.contributor.authorCastro, Paula M.L.
dc.contributor.authorVosátka, Miroslav
dc.contributor.authorDodd, John C.
dc.date.accessioned2011-07-29T09:27:12Z
dc.date.available2011-07-29T09:27:12Z
dc.date.issued2010
dc.description.abstractGenetic, phenotypic and functional variation in a Glomus geosporum isolate obtained from a highly alkaline anthropogenic sediment was investigated. Two cultivation lineages of G. geosporum (BEG199 and BEG211) were created by sub-culturing with or without the stress of the sediment. Genetic variation was assessed on spores from both cultivation lineages in the large sub-unit rRNA gene D2 region using PCR-single strand conformational polymorphism (PCR-SSCP) and sequencing. Phenotypical and functional variation of the cultivation lineages were assessed after inoculation onto Conyza bilbaoana. The sequence diversity obtained in G. geosporum BEG211 was significantly different from that obtained in G. geosporum BEG199. Glomus geosporum BEG199 was more effective than G. geosporum BEG211 in promoting the growth of C. bilbaoana in inert substrate and in increasing its leaf phosphorus concentration when the plant was grown in sediment. After inoculation onto C. bilbaoana, G. geosporum BEG199 colonised the roots to a greater extent, produced a significantly larger number of spores and presented a greater length of extraradical mycelium than G. geosporum BEG211. The results indicate that environmental conditions under which arbuscular mycorrhizal fungi (AMF) are cultivated can influence their effects in host plants. Also, AMF might quickly lose gained-tolerance to environmental stresses when maintained without the selective pressure of those stresses. This study has implications for the production of commercial inoculum of AMF and for the maintenance of AMF cultures. The results indicate that inoculum of AMF should be produced and AMF cultures should be maintained in substrates or media containing the original edaphic stresses.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationOLIVEIRA, Rui S... [et al.] - Genetic, phenotypic and functional variation within a Glomus geosporum isolate cultivated with or without the stress of a highly alkaline anthropogenic sediment. Applied Soil Ecology .ISSN: 0929-1393. Vol. 45, n.º 1 (2010), p. 39-48por
dc.identifier.doi10.1016/j.apsoil.2010.01.008
dc.identifier.eissn1873-0272
dc.identifier.issn0929-1393
dc.identifier.urihttp://hdl.handle.net/10400.14/4846
dc.identifier.wosWOS:000276868300004
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectAMF inoculum productionpor
dc.subjectArbuscular mycorrhizal fungipor
dc.subjectEnvironmental stresspor
dc.subjectGenetic phenotypic and functional variationpor
dc.subjectIntra-isolate variationpor
dc.subjectHighly alkaline sedimentpor
dc.titleGenetic, phenotypic and functional variation within a Glomus geosporum isolate cultivated with or without the stress of a highly alkaline anthropogenic sedimentpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage48
oaire.citation.issue1
oaire.citation.startPage39
oaire.citation.titleApplied Soil Ecology
oaire.citation.volume45
person.familyNameOliveira
person.familyNameCarvalho
person.familyNameCastro
person.familyNameVosátka
person.givenNameRui S.
person.givenNameM. Fátima
person.givenNamePaula
person.givenNameMiroslav
person.identifier2013444
person.identifier.ciencia-id251B-951A-F21E
person.identifier.ciencia-id6913-1464-155B
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.ciencia-id661D-7253-FF86
person.identifier.orcid0000-0001-5252-5595
person.identifier.orcid0000-0002-7181-0540
person.identifier.orcid0000-0001-8841-6606
person.identifier.orcid0000-0002-1378-3185
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id56865275400
person.identifier.scopus-author-id14038677200
person.identifier.scopus-author-id7102781782
person.identifier.scopus-author-id7003642664
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscovery964897d5-02c3-4efe-a8c3-44ea68cf4974

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