Logo do repositório
 
Publicação

Improving Properties of a Novel β-Galactosidase from Lactobacillus plantarum by Covalent Immobilization

2015Artigo científico Acesso restrito
Ver registo simples
dc.contributor.authorBenavente, Rocio
dc.contributor.authorPessela, Benevides C.
dc.contributor.authorCuriel, Jose Antonio
dc.contributor.authorde las Rivas, Blanca
dc.contributor.authorMuñoz, Rosario
dc.contributor.authorGuisán, Jose Manuel
dc.contributor.authorMancheño, Jose M.
dc.contributor.authorCardelle-Cobas, Alejandra
dc.contributor.authorRuiz-Matute, Ana I.
dc.contributor.authorCorzo, Nieves
dc.date.accessioned2016-04-21T16:31:49Z
dc.date.available2016-04-21T16:31:49Z
dc.date.issued2015
dc.description.abstractA novel -galactosidase from Lactobacillus plantarum (LPG) was over-expressed in E. coli and purified via a single chromatographic step by using lowly activated IMAC (immobilized metal for affinity chromatography) supports. The pure enzyme exhibited a high hydrolytic activity of 491 IU/mL towards o-nitrophenyl -d-galactopyranoside. This value was conserved in the presence of different divalent cations and was quite resistant to the inhibition effects of different carbohydrates. The pure multimeric enzyme was stabilized by multipoint and multisubunit covalent attachment on glyoxyl-agarose. The glyoxyl-LPG immobilized preparation was over 20-fold more stable than the soluble enzyme or the one-point CNBr-LPG immobilized preparation at 50 degrees C. This -galactosidase was successfully used in the hydrolysis of lactose and lactulose and formation of different oligosaccharides was detected. High production of galacto-oligosaccharides (35%) and oligosaccharides derived from lactulose (30%) was found and, for the first time, a new oligosaccharide derived from lactulose, tentatively identified as 3'-galactosyl lactulose, has been described.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationBENAVENTE, Rocio ...[et al.] - Improving Properties of a Novel β-Galactosidase from Lactobacillus plantarum by Covalent Immobilization. Molecules. ISSN 1420-3049. Vol. 20 (2015), p. 7874-7889pt_PT
dc.identifier.doi10.3390/molecules20057874
dc.identifier.eid84929191981
dc.identifier.issn1420-3049
dc.identifier.pmid25942370
dc.identifier.urihttp://hdl.handle.net/10400.14/19934
dc.identifier.wos000357157600025
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectβ-galactosidasept_PT
dc.subjectLactobacillus plantarumpt_PT
dc.subjectImmobilizationpt_PT
dc.subjectGlyoxyl-agarosept_PT
dc.subjectOligosaccharides synthesispt_PT
dc.subjectLactosept_PT
dc.subjectLactulosept_PT
dc.titleImproving Properties of a Novel β-Galactosidase from Lactobacillus plantarum by Covalent Immobilizationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage7889
oaire.citation.issue5
oaire.citation.startPage7874
oaire.citation.titleMolecules
oaire.citation.volume20
person.familyNamePessela
person.familyNameCuriel Gamiz
person.familyNamede las Rivas
person.familyNameMuñoz
person.familyNameGuisan
person.familyNameMancheño
person.familyNameCardelle Cobas
person.familyNameRuiz-Matute
person.familyNameCorzo
person.givenNameBenevides
person.givenNameJose Antonio
person.givenNameBlanca
person.givenNameRosario
person.givenNameJose M.
person.givenNameJose Miguel
person.givenNameAlejandra
person.givenNameAna Isabel
person.givenNameNieves
person.identifier279277
person.identifier344696
person.identifier.orcid0000-0003-1747-0124
person.identifier.orcid0000-0002-0111-1437
person.identifier.orcid0000-0002-8357-5226
person.identifier.orcid0000-0003-4170-9713
person.identifier.orcid0000-0003-1627-6522
person.identifier.orcid0000-0001-6943-3291
person.identifier.orcid0000-0002-1271-513X
person.identifier.orcid0000-0001-9143-8128
person.identifier.orcid0000-0002-6420-2344
person.identifier.ridH-8831-2015
person.identifier.ridK-3899-2014
person.identifier.ridH-8597-2012
person.identifier.ridK-4110-2016
person.identifier.ridK-4984-2014
person.identifier.ridK-7183-2017
person.identifier.scopus-author-id16505958800
person.identifier.scopus-author-id9736687700
person.identifier.scopus-author-id13609034100
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3fcca932-f1fd-4ff7-a2f0-1f9c71098b13
relation.isAuthorOfPublication99a0f7dd-b6df-4b81-b1e3-b93de9854304
relation.isAuthorOfPublicationa34fe724-5c84-44ca-a7e7-c45b09bf89eb
relation.isAuthorOfPublication0e003bf2-b994-40aa-a127-827a41f2c5d4
relation.isAuthorOfPublication8ee2d1fa-b4aa-4e12-b4c5-2b22abc05022
relation.isAuthorOfPublicationdba91e4e-5ce2-4527-80f1-9a9cb2704e22
relation.isAuthorOfPublication48ad3ed1-3a70-42fa-b935-e8ef001f6cce
relation.isAuthorOfPublication3d203c9e-f76c-4a87-bbde-c56798ef575e
relation.isAuthorOfPublication747d9866-af06-4a60-8188-311bfe8e4ae2
relation.isAuthorOfPublication.latestForDiscovery48ad3ed1-3a70-42fa-b935-e8ef001f6cce

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
26871289.pdf
Tamanho:
1.36 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
3.44 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CBQF - Contribuições em Revistas Científicas / Contribution to Journals