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β-lactoglobulin micro- and nanostructures as bioactive compounds vehicle: In vitro studies

2020Journal article Open access
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dc.contributor.authorSimões, Lívia S.
dc.contributor.authorMartins, Joana T.
dc.contributor.authorPinheiro, Ana C.
dc.contributor.authorVicente, António A.
dc.contributor.authorRamos, Oscar. L.
dc.date.accessioned2020-01-27T15:01:46Z
dc.date.available2020-01-27T15:01:46Z
dc.date.issued2020
dc.description.abstractβ-Lactoglobulin (β-Lg) is known to be capable to bind hydrophilic and hydrophobic bioactive compounds. This research aimed to assess the in vitro performance of β-Lg micro- (diameter ranging from 200 to 300 nm) and nano (diameter < 100 nm) structures associated to hydrophilic and hydrophobic model compounds on Caco-2 cells and under simulated gastrointestinal (GI) conditions. Riboflavin and quercetin were studied as hydrophilic and hydrophobic model compounds, respectively. Cytotoxicity experiment was conducted using in vitro cellular model based on human colon carcinoma Caco-2 cells. Moreover, the digestion process was simulated using the harmonized INFOGEST in vitro digestion model, where samples were taken at each phase of digestion process - oral, gastric and intestinal - and characterized in terms of particle size, polydispersity index (PDI), surface charge by dynamic light scattering (DLS); protein hydrolysis degree by 2,4,6-trinitrobenzene sulfonic acid (TNBSA) assay and native polyacrylamide gel electrophoresis; and bioactive compound concentration. Caco-2 cell viability was not affected up to 21 × 10−3 mg mL−1 of riboflavin and 16 × 10−3 mg mL−1 quercetin on β-Lg micro- and nanostructures. In the oral phase, β-Lg structures’ particle size, PDI and surface charge values were not changed comparing to the initial β-Lg structures (i.e., before being subjected to in vitro GI digestion). During gastric digestion, β-Lg structures were resistant to proteolytic enzymes and to acid environment of the stomach – confirmed by TNBSA and native gel electrophoresis. In vitro digestion results indicated that β-Lg micro- and nanostructures protected both hydrophilic and hydrophobic compounds from gastric conditions and deliver them to target site (i.e., intestinal phase). In addition, β-Lg structures were capable to enhance riboflavin and quercetin bioaccessibility and bioavailability potential compared to bioactive compounds in their free form. This study indicated that β-Lg micro- and nanostructures were capable to enhance hydrophilic and hydrophobic compounds bioavailability potential and they can be used as oral delivery systems.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationSimões, L. S., Martins, J. T., Pinheiro, A. C., Vicente, A. A., & Ramos, O. L. (2020). β-lactoglobulin micro-and nanostructures as bioactive compounds vehicle: In vitro studies. Food Research International, 131: 108979pt_PT
dc.identifier.doi10.1016/j.foodres.2020.108979pt_PT
dc.identifier.eid85077700746
dc.identifier.issn0963-9969
dc.identifier.pmid32247463
dc.identifier.urihttp://hdl.handle.net/10400.14/29351
dc.identifier.wos000528252800045
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPOCI/01/0145/FEDER/006684pt_PT
dc.relationNORTE/01/0145/FEDER/000004pt_PT
dc.relationFCOMP/01/0124/FEDER/027462pt_PT
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBioaccessibilitypt_PT
dc.subjectBioavailabilitypt_PT
dc.subjectCaco-2 cellspt_PT
dc.subjectDelivery systemspt_PT
dc.subjectHydrophilic compoundspt_PT
dc.subjectHydrophobic compoundspt_PT
dc.subjectFood-gradept_PT
dc.titleβ-lactoglobulin micro- and nanostructures as bioactive compounds vehicle: In vitro studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04469%2F2013/PT
oaire.citation.titleFood Research Internationalpt_PT
oaire.citation.volume131pt_PT
oaire.fundingStream5876
person.familyNameSimões
person.familyNameMartins
person.familyNamePinheiro
person.familyNameVicente
person.familyNameRamos
person.givenNameLivia
person.givenNameJoana
person.givenNameAna C
person.givenNameAntónio
person.givenNameOscar
person.identifier464583
person.identifier175018
person.identifier.ciencia-id7316-13C1-59A5
person.identifier.ciencia-id4B12-CD33-75EE
person.identifier.ciencia-idB112-A77C-62CC
person.identifier.ciencia-id5C19-49E0-96EF
person.identifier.orcid0000-0002-9083-8709
person.identifier.orcid0000-0003-1166-522X
person.identifier.orcid0000-0002-8223-3742
person.identifier.orcid0000-0003-3593-8878
person.identifier.orcid0000-0002-7627-189X
person.identifier.ridH-3799-2013
person.identifier.ridO-6034-2015
person.identifier.ridH-1555-2013
person.identifier.ridM-3981-2013
person.identifier.scopus-author-id57193858057
person.identifier.scopus-author-id35424163100
person.identifier.scopus-author-id23098217400
person.identifier.scopus-author-id7005820757
person.identifier.scopus-author-id54889028400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationde500504-c78c-45ae-9e6b-a4e3066d7eda
relation.isAuthorOfPublicationd95f2954-dc08-46a9-af7d-15052578910e
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relation.isAuthorOfPublication.latestForDiscovery09f80cea-4d6b-445b-9c06-866dc6cc0dfb
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