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Efficient screening and enhanced exopolysaccharide production by functional lactic acid bacteria (LAB) in lactose supplemented media

2024-01-17Artigo de investigação Acesso aberto
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dc.contributor.authorPintado, Ana I. E.
dc.contributor.authorBarbosa, Catarina C. R.
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorMalcata, F. Xavier
dc.contributor.authorGomes, Ana M. P.
dc.date.accessioned2026-01-27T13:00:18Z
dc.date.available2026-01-27T13:00:18Z
dc.date.issued2024-01-17
dc.description.abstractExopolysaccharides (EPS) produced by lactic acid bacteria (LAB) can be considered as natural biological thickeners that have attracted considerable attention in the food industry. This study aimed to evaluate and select potentially EPS-producing strains LAB and to assess the influence of carbon source and aeration on EPS production. Nine LAB strains were assessed as potential EPS producers, and Rahnella aquatilis ATCC 55046 was employed as the positive control strain for EPS. The compaction test and the observation of viscous colonies in a solid medium did not yield sufficient evidence for the presence of EPS. The assessment of capsules through staining provided evidence of EPS presence only for Rahnella aquatilis ATCC 55046. The EPS yield was subsequently assessed in De-Man Rogosa and Sharpe (MRS) broth medium supplemented with 2% (w/w) fructose (MRS-f) or lactose (MRS-l), as well as in whey (Whey) and whey supplemented with 2% (w/w) lactose (Whey-l). The EPS production in the various culture media under study ranged from 194 to 1,187 mg of EPS/g of polymer dry mass (PDM). These results suggest that the culture medium and carbon sources had an impact on the EPS production of the different strains. Bifidobacterium animalis Bb12 achieved the highest EPS production in MRS-f. In the case of MRS-l, the control strain recorded the highest EPS value, along with Lactobacillus acidophilus LAC-1. Regarding Whey, Lentilactobacillus Kefir NCFB 2753 exhibited the highest EPS production, while in Whey-l, Lacticaseibacillus paracasei LCS-1 emerged as the top performer in terms of EPS production. This suggests that certain strains exhibit potential for use in the production of novel fermented EPS products, whether dairy or non-dairy.eng
dc.identifier.citationPintado, A. I. E., Barbosa, C. C. R., Pintado, M. E., & Malcata, F. X. et al. (2024). Efficient screening and enhanced exopolysaccharide production by functional lactic acid bacteria (LAB) in lactose supplemented media. Applied Microbiology: Theory ? Technology, 5(1), 37-50. https://doi.org/10.37256/amtt.5120243374
dc.identifier.doi10.37256/amtt.5120243374
dc.identifier.issn2717-5928
dc.identifier.other3bbbe6c7-2759-4b37-ae8d-c59d5e192f79
dc.identifier.urihttp://hdl.handle.net/10400.14/56789
dc.language.isoeng
dc.peerreviewedyes
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBovine whey
dc.subjectCarbon sources
dc.subjectEPS screening
dc.subjectExopolysaccharides
dc.subjectLactic acid bacteria
dc.subjectMRS broth
dc.titleEfficient screening and enhanced exopolysaccharide production by functional lactic acid bacteria (LAB) in lactose supplemented mediaeng
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.endPage50
oaire.citation.issue1
oaire.citation.startPage37
oaire.citation.titleApplied Microbiology: Theory ? Technology
oaire.citation.volume5
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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