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Method for bacteriophage isolation against target campylobacter strains

2010Journal article Restricted access
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dc.contributor.authorCarvalho, C.
dc.contributor.authorSusana, M.
dc.contributor.authorFernandes, E.
dc.contributor.authorSantos, S.
dc.contributor.authorGannon, B.
dc.contributor.authorNicolau, A.
dc.contributor.authorGibbs, P.
dc.contributor.authorTeixeira, P.
dc.contributor.authorAzeredo, J.
dc.date.accessioned2010-10-06T12:14:09Z
dc.date.available2010-10-06T12:14:09Z
dc.date.issued2010
dc.description.abstractAims: Poultry meat is considered a major source of Campylobacter. This micro-aerobic bacterium is commonly responsible for foodborne illness. This work focuses on the isolation of Campylobacter coli lytic bacteriophages (phages) against target C. coli strains. Methods and Results: A method involving the enrichment of free-range chicken samples in a broth containing the target C. coli strains and salts (CaCl2 and MgSO4) was used for phage isolation. This method allowed the isolation of 43 phages that were active against 83% of the C. coli strains used in the isolation procedure. Approximately 65% of the phages were also effective against Campylobacter jejuni strains. Conclusions: The use of target pathogens in the phage isolation step improves the likelihood of detecting and isolating phages for the control of these specific strains. Significance and Impact of the Study: This technique will be valuable in the context of phage therapy for enriching for phages that are active against specifically identified strains of bacteria, for example from a food poisoning outbreak or epidemic strains resistant to multiple antibiotics. In these situations, using the conventional methods for searching for bacteriophages active for these particular strains can be a time-consuming, if not an unsuccessful process. Using the isolation method described in this manuscript, the particular strains can be added to the enrichment broth increasing the probability of finding phages against them. Therefore, it will shorten the time needed for seeking phages able to lyse target strains, which in most of the cases, because of the rapid increase in antimicrobial-resistant bacteria, is of crucial importance.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citation"Letters in Applied Microbiology". ISSN 0266-8254. 50, nº2 (2010) 192–197por
dc.identifier.doi10.1111/j.1472-765X.2009.02774.x
dc.identifier.eissn1472-765X
dc.identifier.issn0266-8254
dc.identifier.urihttp://hdl.handle.net/10400.14/2664
dc.identifier.wosWOS:000273316800010
dc.language.isoengpor
dc.publisherThe Society for Applied Microbiologypor
dc.relationFP-6-2003-Food-2-A
dc.subjectBacteriophagepor
dc.subjectCampylobacter coli,por
dc.subjectCampylobacter jejunipor
dc.subjectIsolationpor
dc.subjectMethodpor
dc.subjectPoultrypor
dc.titleMethod for bacteriophage isolation against target campylobacter strainspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F23484%2F2005/PT
oaire.citation.endPage197
oaire.citation.issue2
oaire.citation.startPage192
oaire.citation.titleLetters in Applied Microbiology
oaire.citation.volume50
oaire.fundingStreamSFRH
person.familyNameCarvalho
person.familyNameFernandes
person.familyNameSantos
person.familyNameNicolau
person.familyNameGibbs
person.familyNameTeixeira
person.familyNameAzeredo
person.givenNameCarla
person.givenNameElisabete
person.givenNameSílvio B
person.givenNameAna
person.givenNamePaul
person.givenNamePaula
person.givenNameJoana
person.identifier.ciencia-id7812-2CED-0B82
person.identifier.ciencia-id8B11-15E7-4F5D
person.identifier.ciencia-idFC11-952D-0675
person.identifier.orcid0000-0002-9345-6546
person.identifier.orcid0000-0003-4558-7047
person.identifier.orcid0000-0003-0801-8080
person.identifier.orcid0000-0001-5012-2258
person.identifier.orcid0000-0002-6580-4363
person.identifier.orcid0000-0002-6296-5137
person.identifier.orcid0000-0002-5180-7133
person.identifier.ridB-8509-2011
person.identifier.ridO-5491-2015
person.identifier.ridJ-2888-2013
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id55931721600
person.identifier.scopus-author-id33467930800
person.identifier.scopus-author-id7006762346
person.identifier.scopus-author-id7005895206
person.identifier.scopus-author-id6701433514
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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