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Advisor(s)
Abstract(s)
This study reports on the intestinal permeability of salt-containing mixtures of amino acids extracted from
codfish salting wastewater. Permeability was evaluated in vitro using the Caco-2 cell line model; cell integrity
during exposure to mixtures of amino acids was estimated by measuring the transepithelial electrical
resistance (TEER). The effect of salt (NaCl) on the permeability and on the intestinal cell’s integrity was
also examined. Permeation rate (i.e. transport) was ≥95% for all amino acids except for creatine, for
which it was 6%. Values for apparent permeability coefficients, Papp > 10
5 cm s
1, for mixture with isotonic
concentration of NaCl suggest that amino acids are very likely to be absorbed in humans. Mixture
with a hypertonic level of NaCl exerts a cytotoxic effect in intestinal cells resulting in a loss of epithelium
integrity. Results show that isotonic mixture of amino acids extracted from codfish salting wastewater
could be used in food, feed, cosmetic and pharmaceutical formulations. These applications could contribute
to the fish industry sustainability.
Description
Keywords
Absorption Amino acids Caco-2 cell Codfish Small intestine
Citation
FERRARO, Vincenza ...[et al.] - In vitro intestinal absorption of amino acid mixtures extracted from codfish (Gadus morhua L.) salting wastewater. International Journal of Food Science and Technology. ISSN 1365-2621. Vol. 49 (2014), p. 27–33
