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Flow-based method for the determination of biomarkers urea and ammoniacal nitrogen in saliva

2020-04-28Journal article Restricted access
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dc.contributor.authorThepchuay, Yanisa
dc.contributor.authorCosta, Carolina F F A
dc.contributor.authorMesquita, Raquel B R
dc.contributor.authorSampaio-Maia, Benedita
dc.contributor.authorNacapricha, Duangjai
dc.contributor.authorRangel, António O. S. S.
dc.date.accessioned2020-05-04T16:34:06Z
dc.date.available2020-05-04T16:34:06Z
dc.date.issued2020-04-28
dc.description.abstractSalivary urea and ammonium levels are potential biomarkers for chronic kidney disease. A fast and efficient assessment of these compounds in the saliva of healthy and diseased individuals may be a useful tool to monitor kidney function. Materials & methods: Ammonium ions were measured with an ammonia selective electrode after conversion to ammonia gas. A urease reactor was incorporated in the manifold to hydrolyze urea to ammonium, thereby providing values of ammonia from both urea and ammonium ions in the sample. The accuracy of the method was assessed by comparison with a commercially available kit for urea and ammonium determination. Conclusion: A sequential injection method for the biparametric determination of salivary urea and ammonium employing a single sequential injection manifold was successfully applied to samples collected from both healthy volunteers and chronic kidney disease patients.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationThepchuay, Y, Costa, C.F., Mesquita, R.B., Sampaio-Maia, B., Nacapricha, D., Rangel, A.O. (2020) Flow-based method for the determination of biomarkers urea and ammoniacal nitrogen in saliva. Bioanalysispt_PT
dc.identifier.doi10.4155/bio-2020-0036pt_PT
dc.identifier.eid85084379951
dc.identifier.issn1757-6180
dc.identifier.pmid32343172
dc.identifier.urihttp://hdl.handle.net/10400.14/30508
dc.identifier.wos000539047700004
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNewlands Presspt_PT
dc.relationRoyal Thai Government Scholarship, Ministry of Higher Education, Science, Research and Innovationpt_PT
dc.relationCentre for Biotechnology and Fine Chemistry
dc.subjectAmmonia selective electrodept_PT
dc.subjectAmmoniumpt_PT
dc.subjectImmobilized urease reactorpt_PT
dc.subjectSalivapt_PT
dc.subjectUreapt_PT
dc.titleFlow-based method for the determination of biomarkers urea and ammoniacal nitrogen in salivapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2019/PT
oaire.citation.titleBioanalysispt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMesquita
person.familyNameSantos Silva Rangel
person.givenNameRaquel
person.givenNameAntónio Osmaro
person.identifierJ-4630-2013
person.identifier.ciencia-idEB1D-DEE3-0B60
person.identifier.ciencia-idD61D-7CFB-178A
person.identifier.orcid0000-0001-7745-1860
person.identifier.orcid0000-0002-6486-8947
person.identifier.ridJ-4630-2013
person.identifier.scopus-author-id7005556626
person.identifier.scopus-author-id7004827387
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication467be022-fd9c-4549-aa48-48ebcc1098eb
relation.isAuthorOfPublication.latestForDiscoveryf89887f1-4d1b-438b-8f03-27d9bda1de7b
relation.isProjectOfPublication1f76a7db-2520-482f-b56b-7e01ea36dd1f
relation.isProjectOfPublication.latestForDiscovery1f76a7db-2520-482f-b56b-7e01ea36dd1f

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