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Advisor(s)
Abstract(s)
Salivary urea and ammonium levels are potential biomarkers for chronic kidney disease. A fast and
efficient assessment of these compounds in the saliva of healthy and diseased individuals may be a useful
tool to monitor kidney function. Materials & methods: Ammonium ions were measured with an ammonia
selective electrode after conversion to ammonia gas. A urease reactor was incorporated in the manifold
to hydrolyze urea to ammonium, thereby providing values of ammonia from both urea and ammonium
ions in the sample. The accuracy of the method was assessed by comparison with a commercially available
kit for urea and ammonium determination. Conclusion: A sequential injection method for the biparametric
determination of salivary urea and ammonium employing a single sequential injection manifold
was successfully applied to samples collected from both healthy volunteers and chronic kidney disease
patients.
Description
Keywords
Ammonia selective electrode Ammonium Immobilized urease reactor Saliva Urea
Pedagogical Context
Citation
Thepchuay, Y, Costa, C.F., Mesquita, R.B., Sampaio-Maia, B., Nacapricha, D., Rangel, A.O. (2020) Flow-based method for the determination of biomarkers urea and ammoniacal nitrogen in saliva. Bioanalysis
Publisher
Newlands Press