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Evaluation of characteristics of Pediococcus spp. to be used as a vaginal probiotic

2013Journal article Restricted access
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dc.contributor.authorBorges, S.
dc.contributor.authorBarbosa, J.
dc.contributor.authorSilva, Joana
dc.contributor.authorTeixeira, Paula
dc.date.accessioned2014-05-08T15:09:45Z
dc.date.available2014-05-08T15:09:45Z
dc.date.issued2013
dc.description.abstractThe aim of our research was to select, identify and characterize an isolate of lactic acid bacteria to be considered as a vaginal probiotic. Methods and Results: Thirty-five isolates of Pediococcus spp. showed bacteriocinogenic activity against Listeria monocytogenes and the ability to survive in simulated vaginal fluid (SVF) at pH 4!2. One isolate of Pediococcus spp. was selected and characterized to evaluate its safety before the use as vaginal probiotic. Pediococcus pentosaceus SB83 did not show the presence of virulence factors such as the production of gelatinase, lipase and DNase, haemolytic activity, nor the presence of virulence genes (genes esp, agg, gelE, efaAfm, efaAfs, cylA, cylB and cylM). Pediococcus pentosaceus SB83 was considered sensitive to chloramphenicol, gentamicin, streptomycin, kanamycin, erythromycin and ampicillin. This strain was considered resistant to tetracycline and vancomycin. Pediococcus pentosaceus SB83 was a biofilm producer at different pH values (4!2, 5!5 and 6!5) in SVF and in de Man, Rogosa and Sharpe medium. Conclusions: The in vitro results provide a basis for the use of P. pentosaceus SB83 as a vaginal probiotic, to prevent colonization by L. monocytogenes in pregnant women. Significance and Impact of Study: The application of vaginal probiotics could have the potential for preventing vaginal infections and consequently reduce abortion and neonatal infections.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationBORGES, S....[et al.] - Evaluation of characteristics of Pediococcus spp. to be used as a vaginal probiotic. Journal of Applied Microbiology. ISSN 1364-5072. Vol. 115 (2013), 527-538por
dc.identifier.doi10.1111/jam.12232
dc.identifier.eissn1365-2672
dc.identifier.issn1364-5072
dc.identifier.urihttp://hdl.handle.net/10400.14/14338
dc.identifier.wosWOS:000321894900021
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.relationStrategic Project - LA 16 - 2011-2012
dc.subjectAntibiotic susceptibilitypor
dc.subjectAntimicrobial activitypor
dc.subjectBiofilmspor
dc.subjectListeria monocytogenespor
dc.subjectPregnancypor
dc.subjectVaginal fluidpor
dc.subjectVaginal probioticpor
dc.subjectVirulence factorspor
dc.titleEvaluation of characteristics of Pediococcus spp. to be used as a vaginal probioticpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 16 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F45496%2F2008/PT
oaire.citation.endPage538
oaire.citation.startPage527
oaire.citation.titleJournal of Applied Microbiology
oaire.citation.volume115
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNameBorges
person.familyNameBarbosa
person.familyNameSilva
person.familyNameTeixeira
person.givenNameSandra
person.givenNameJoana
person.givenNameJoana
person.givenNamePaula
person.identifier.ciencia-id591F-66D5-8F90
person.identifier.ciencia-id9516-589F-8B0D
person.identifier.ciencia-idB415-743D-D9F9
person.identifier.orcid0000-0003-4665-1098
person.identifier.orcid0000-0001-5879-9458
person.identifier.orcid0000-0003-3977-7418
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id15826021600
person.identifier.scopus-author-id13608752600
person.identifier.scopus-author-id7403023130
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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