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Advisor(s)
Abstract(s)
The aim of our research was to select, identify and characterize an
isolate of lactic acid bacteria to be considered as a vaginal probiotic.
Methods and Results: Thirty-five isolates of Pediococcus spp. showed
bacteriocinogenic activity against Listeria monocytogenes and the ability to
survive in simulated vaginal fluid (SVF) at pH 4!2. One isolate of Pediococcus
spp. was selected and characterized to evaluate its safety before the use as
vaginal probiotic. Pediococcus pentosaceus SB83 did not show the presence of
virulence factors such as the production of gelatinase, lipase and DNase,
haemolytic activity, nor the presence of virulence genes (genes esp, agg, gelE,
efaAfm, efaAfs, cylA, cylB and cylM). Pediococcus pentosaceus SB83 was
considered sensitive to chloramphenicol, gentamicin, streptomycin, kanamycin,
erythromycin and ampicillin. This strain was considered resistant to
tetracycline and vancomycin. Pediococcus pentosaceus SB83 was a biofilm
producer at different pH values (4!2, 5!5 and 6!5) in SVF and in de Man,
Rogosa and Sharpe medium.
Conclusions: The in vitro results provide a basis for the use of P. pentosaceus
SB83 as a vaginal probiotic, to prevent colonization by L. monocytogenes in
pregnant women.
Significance and Impact of Study: The application of vaginal probiotics could
have the potential for preventing vaginal infections and consequently reduce
abortion and neonatal infections.
Description
Keywords
Antibiotic susceptibility Antimicrobial activity Biofilms Listeria monocytogenes Pregnancy Vaginal fluid Vaginal probiotic Virulence factors
Citation
BORGES, S....[et al.] - Evaluation of characteristics of Pediococcus spp. to be used as a vaginal probiotic. Journal of Applied Microbiology. ISSN 1364-5072. Vol. 115 (2013), 527-538
