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Sequential injection kinetic flow assay for monitoring glycerol in a sugar fermentation process by saccharomyces cerevisiae

2010Journal article Restricted access
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dc.contributor.authorHueso Domínguez, Karina B.
dc.contributor.authorTóth, Ildikó V.
dc.contributor.authorSouto, M. Renata S.
dc.contributor.authorMendes, Filipa
dc.contributor.authorGarcía De María, Cándido
dc.contributor.authorVasconcelos, Isabel
dc.contributor.authorRangel, António O. S. S.
dc.date.accessioned2010-10-06T15:07:38Z
dc.date.available2010-10-06T15:07:38Z
dc.date.issued2010
dc.description.abstractA sequential injection system to monitor glycerol in a Saccharomyces cerevisiae fermentation process was developed. The method relies on the rate of formation of nicotinamide adenine dinucleotide in its reduced form (NADH, measured spectrophotometrically at 340 nm) from the reaction of glycerol with NAD+ cofactor, catalysed by the enzyme glycerol dehydrogenase present in solution. This procedure enables the determination of glycerol between 0.046 and 0.46 g/l, (corresponding to yeast fermentation samples with concentrations up to 50 g/l) with good repeatability (relative standard deviation for n = 10 lower than 2.2% for three different samples) at a sampling frequency of 25/h. The detection and quantification limits using a miniaturised spectrophotometer were 0.13 and 0.44 mM, respectively. Reagent consumption was of 0.45 μmol NAD+ and 1.8 μg enzyme per assay, and the waste production was 2.8 ml per determination. Results obtained for samples were in agreement with those obtained with a high-performance liquid chromatography method.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citation"Applied Biochemistry and Biotechnology" . ISSN 1559-0291. 160: 6 (2010) 1664-1673por
dc.identifier.doi10.1007/s12010-009-8675-z
dc.identifier.eissn1559-0291
dc.identifier.issn0273-2289
dc.identifier.urihttp://hdl.handle.net/10400.14/2669
dc.identifier.wosWOS:000274614400008
dc.language.isoengpor
dc.publisherSpringer Verlagpor
dc.relationBQU2002-02332
dc.subjectSequential injection analysispor
dc.subjectGlycerolpor
dc.subjectGlucose fermentationpor
dc.subjectSaccharomyces cerevisiaepor
dc.subjectKinetic methodpor
dc.subjectEnzymatic assaypor
dc.titleSequential injection kinetic flow assay for monitoring glycerol in a sugar fermentation process by saccharomyces cerevisiaepor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F5631%2F2001/PT
oaire.citation.endPage1673
oaire.citation.issue6
oaire.citation.startPage1664
oaire.citation.titleApplied Biochemistry and Biotechnology
oaire.citation.volume160
oaire.fundingStreamSFRH
person.familyNameHueso
person.familyNameTóth
person.familyNameSouto
person.familyNameVasconcelos
person.familyNameSantos Silva Rangel
person.givenNameKarina
person.givenNameIldikó
person.givenNameRenata
person.givenNameIsabel
person.givenNameAntónio Osmaro
person.identifier.ciencia-id9814-DF91-15FA
person.identifier.ciencia-id851B-41B9-4746
person.identifier.ciencia-idD61D-7CFB-178A
person.identifier.orcid0000-0001-9329-9037
person.identifier.orcid0000-0002-7342-1063
person.identifier.orcid0000-0002-2130-8667
person.identifier.orcid0000-0002-0565-3516
person.identifier.orcid0000-0002-6486-8947
person.identifier.ridG-1733-2010
person.identifier.ridM-7017-2013
person.identifier.ridN-3870-2013
person.identifier.scopus-author-id35512332800
person.identifier.scopus-author-id7005270435
person.identifier.scopus-author-id7003767794
person.identifier.scopus-author-id7004827387
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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