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Cytotoxicity induced by extracts of pisolithus tinctorius spores on human cancer and normal cell lines - evaluation of the anticancer potential

2015Journal article Restricted access
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dc.contributor.authorAlves, Ricardo
dc.contributor.authorPreto, Marco
dc.contributor.authorVasconcelos, Vitor
dc.contributor.authorOliveira, Rui
dc.contributor.authorMartins, Rosário
dc.date.accessioned2016-04-21T15:08:03Z
dc.date.available2016-04-21T15:08:03Z
dc.date.issued2015
dc.description.abstractFungi have been considered a potential source of natural anticancer drugs. However, studies on these organisms have mainly focused on compounds present in the sporocarp and mycelium. The aim of this study was to assess the anticancer potential of fungal spores using a bioassay-guided fractionation with cancer and normal cell lines. Crude extracts from spores of the basidiomycetous fungus Pisolithus tinctorius were prepared using five solvents/solvent mixtures in order to select the most effective crude extraction procedure. A dichloromethane/methanol (DCM/MeOH) mixture was found to produce the highest extraction yield, and this extract was fractionated into 11 fractions. Crude extracts and fractions were assayed for cytotoxicity in the human osteocarcinoma cell line MG63, the human breast carcinoma cell line T47D, the human colon adenocarcinoma cell line RKO, and the normal human brain capillary endothelial cell line hCMEC/D3. Cytotoxicity was assessed by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reduction assay. The results showed a reduction in cancer cell viability of approximately 95% with 4 of 11 fractions without a significant reduction in viability of hCMEC/D3 cells. Data demonstrated that spores of P. tinctorius might serve as an interesting source of compounds with potential anticancer properties.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationALVES, Ricardo; PRETO, Marco; VASCONCELOS, Vitor; OLIVEIRA, Rui; MARTINS, Rosário - Cytotoxicity Induced by Extracts of Pisolithus tinctorius Spores on Human Cancer and Normal Cell Lines—Evaluation of the Anticancer Potential. Journal of Toxicology and Environmental Health. ISSN 1528-7394. Part A, Vol. 78 (2015), p 840–847pt_PT
dc.identifier.doi10.1080/15287394.2015.1051176
dc.identifier.eid84942112595
dc.identifier.eissn1087-2620
dc.identifier.issn1528-7394
dc.identifier.pmid26167750
dc.identifier.urihttp://hdl.handle.net/10400.14/19911
dc.identifier.wos000357938400006
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francis Inc
dc.relationNORTE-07-0124-FEDER-000047
dc.titleCytotoxicity induced by extracts of pisolithus tinctorius spores on human cancer and normal cell lines - evaluation of the anticancer potentialpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F85008%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FMAR%2FLA0015%2F2013/PT
oaire.citation.endPage847
oaire.citation.issue13-14
oaire.citation.startPage840
oaire.citation.titleJournal of Toxicology and Environmental Health, Part A: Current Issues
oaire.citation.volume78
oaire.fundingStreamSFRH
oaire.fundingStreamCOMPETE
person.familyNameCorreia Preto
person.familyNameVasconcelos
person.familyNameOliveira
person.familyNameMartins
person.givenNameMarco Aurélio
person.givenNameVitor
person.givenNameRui S.
person.givenNameRosario
person.identifier162986
person.identifier.ciencia-id971A-DE59-B76D
person.identifier.ciencia-idF81D-B3FB-B39C
person.identifier.ciencia-id251B-951A-F21E
person.identifier.ciencia-idA919-DB40-EF10
person.identifier.orcid0000-0002-9697-5579
person.identifier.orcid0000-0003-3585-2417
person.identifier.orcid0000-0001-5252-5595
person.identifier.orcid0000-0002-9882-4651
person.identifier.ridA-8933-2008
person.identifier.scopus-author-id7004649080
person.identifier.scopus-author-id56865275400
person.identifier.scopus-author-id21834368700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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