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The chemopreventive effect of the dietary compound kaempferol on the MCF-7 human breast cancer cell line is dependent on inhibition of glucose cellular uptake

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dc.contributor.authorAzevedo, Cláudia
dc.contributor.authorCorreia-Branco, Ana
dc.contributor.authorAraújo, João R.
dc.contributor.authorGuimarães, João T.
dc.contributor.authorKeating, Elisa
dc.contributor.authorMartel, Fátima
dc.date.accessioned2015-05-11T10:31:35Z
dc.date.available2015-05-11T10:31:35Z
dc.date.issued2015
dc.description.abstractOur aim was to investigate the effect of several dietary polyphenols on glucose uptake by breast cancer cells. Uptake of H-3-deoxy-D-glucose (H-3-DG) by MCF-7 cells was time-dependent, saturable, and inhibited by cytochalasin B plus phloridzin. In the short-term (26min), myricetin, chrysin, genistein, resveratrol, kaempferol, and xanthohumol (10-100 mu M) inhibited H-3-DG uptake. Kaempferol was found to be the most potent inhibitor of H-3-DG uptake [IC50 of 4 mu M (1.6-9.8)], behaving as a mixed-type inhibitor. In the long-term (24h), kaempferol (30 mu M) was also able to inhibit H-3-DG uptake, associated with a 40% decrease in GLUT1 mRNA levels. Interestingly enough, kaempferol (100 mu M) revealed antiproliferative (sulforhodamine B and H-3-thymidine incorporation assays) and cytotoxic (extracellular lactate dehydrogenase activity determination) properties, which were mimicked by low extracellular (1mM) glucose conditions and reversed by high extracellular (20mM) glucose conditions. Finally, exposure of cells to kaempferol (30 mu M) induced an increase in extracellular lactate levels over time (to 731 +/- 32% of control after a 24 h exposure), due to inhibition of MCT1-mediated lactate cellular uptake. In conclusion, kaempferol potently inhibits glucose uptake by MCF-7 cells, apparently by decreasing GLUT1-mediated glucose uptake. The antiproliferative and cytotoxic effect of kaempferol in these cells appears to be dependent on this effect.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationAZEVEDO, Cláudia ; CORREIA-BRANCO, Ana ; ARAÚJO, João R. ; GUIMARÃES, João T. ; KEATING, Elisa ; MARTEL, Fátima - The Chemopreventive Effect of the Dietary Compound Kaempferol on the MCF-7 Human Breast Cancer Cell Line Is Dependent on Inhibition of Glucose Cellular Uptake. Nutrition and Cancer. ISSN 0163-5581. (2015) vol. 67, nº 3, p.504-513por
dc.identifier.doi10.1080/01635581.2015.1002625
dc.identifier.eid84926523586
dc.identifier.eissn1532-7914
dc.identifier.issn0163-5581
dc.identifier.pmid25719685
dc.identifier.urihttp://hdl.handle.net/10400.14/17530
dc.identifier.wos000352316200015
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherTaylor & Francis
dc.titleThe chemopreventive effect of the dietary compound kaempferol on the MCF-7 human breast cancer cell line is dependent on inhibition of glucose cellular uptakepor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-OSM%2F102239%2F2008/PT
oaire.citation.endPage513
oaire.citation.issue3
oaire.citation.startPage504
oaire.citation.titleNutrition and Cancer
oaire.citation.volume67
oaire.fundingStream5876-PPCDTI
person.familyNameAzevedo
person.familyNameGuimarães
person.familyNameKeating
person.familyNameMartel
person.givenNameCláudia
person.givenNameJoão Tiago
person.givenNameElisa
person.givenNameFátima
person.identifier517090
person.identifier.ciencia-idE817-6AD8-E6C3
person.identifier.ciencia-id2E1C-FAA4-A661
person.identifier.ciencia-id5C14-37B4-FDCA
person.identifier.orcid0000-0002-7750-1888
person.identifier.orcid0000-0003-4836-6311
person.identifier.orcid0000-0002-3904-9907
person.identifier.orcid0000-0002-0525-3416
person.identifier.ridT-3079-2017
person.identifier.scopus-author-id7102733078
person.identifier.scopus-author-id35612071300
person.identifier.scopus-author-id7005164101
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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