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Differential lipid accumulation on HepG2 cells triggered by palmitic and linoleic fatty acids exposure

2023-03-04Journal article Open access
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dc.contributor.authorTeixeira, Francisca S.
dc.contributor.authorPimentel, Lígia L.
dc.contributor.authorVidigal, Susana S. M. P.
dc.contributor.authorAzevedo-Silva, João
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorRodríguez-Alcalá, Luís M.
dc.date.accessioned2023-03-06T17:24:42Z
dc.date.available2023-03-06T17:24:42Z
dc.date.issued2023-03-04
dc.description.abstractLipid metabolism pathways such as β-oxidation, lipolysis and, lipogenesis, are mainly associated with normal liver function. However, steatosis is a growing pathology caused by the accumulation of lipids in hepatic cells due to increased lipogenesis, dysregulated lipid metabolism, and/or reduced lipolysis. Accordingly, this investigation hypothesizes a selective in vitro accumulation of palmitic and linoleic fatty acids on hepatocytes. After assessing the metabolic inhibition, apoptotic effect, and reactive oxygen species (ROS) generation by linoleic (LA) and palmitic (PA) fatty acids, HepG2 cells were exposed to different ratios of LA and PA to study the lipid accumulation using the lipophilic dye Oil Red O. Lipidomic studies were also carried out after lipid isolation. Results revealed that LA was highly accumulated and induced ROS production when compared to PA. Lipid profile modifications were observed after LA:PA 1:1 (v/v) exposure, which led to a four-fold increase in triglycerides (TGs) (mainly in linoleic acid-containing species), as well as a increase in cholesterol and polyunsaturated fatty acids (PUFA) content when compared to the control cells. The present work highlights the importance of balancing both PA and LA fatty acids concentrations in HepG2 cells to maintain normal levels of free fatty acids (FFAs), cholesterol, and TGs and to minimize some of the observed in vitro effects (i.e., apoptosis, ROS generation and lipid accumulation) caused by these fatty acids.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/molecules28052367pt_PT
dc.identifier.eid85149883552
dc.identifier.issn1420-3049
dc.identifier.pmid36903612
dc.identifier.urihttp://hdl.handle.net/10400.14/40456
dc.identifier.wos000947962000001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSteatosispt_PT
dc.subjectHepatocytespt_PT
dc.subjectFatty acidspt_PT
dc.subjectTriglyceridespt_PT
dc.subjectOil Red Opt_PT
dc.subjectLipid accumulationpt_PT
dc.titleDifferential lipid accumulation on HepG2 cells triggered by palmitic and linoleic fatty acids exposurept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue5pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume28pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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