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Development of a sustainable bioprocess based on green technologies for xylitol production from corn cob

dc.contributor.authorBaptista, Sara L.
dc.contributor.authorCarvalho, Luís
dc.contributor.authorRomaní, Aloia
dc.contributor.authorDomingues, Lucília
dc.date.accessioned2021-01-11T17:36:36Z
dc.date.available2021-01-11T17:36:36Z
dc.date.issued2020
dc.description.abstractIn this work, a sustainable and environmental friendly strategy for the biotechnological production of xylitol was proposed and optimized. For this purpose, corn cob was hydrothermally pretreated at high solid loadings (25%) for an efficient solubilization of xylan in hemicellulose derived compounds, xylooligosaccharides and xylose. Xylose enriched streams were obtained from the enzymatic saccharification of the whole slurry (solid and liquid fraction) resulting from the autohydrolysis pretreatment. The xylitol production in a simultaneous saccharification and fermentation (SSF) process, by the recombinant Saccharomyces cerevisiae PE-2-GRE3 strain, was optimized using different enzyme and substrate (pretreated corn cob solid) loadings by an experimental design. This study demonstrated a significant effect of substrate loading on the production process achieving a maximal concentration of 47 g/L with 6.7 % of pretreated corn cob and 24 FPU/g of enzyme loading, with partial detoxification of the hydrolysate. Furthermore, the 1.42-fold increase in xylitol titer and 1.56-fold increase in productivity achieved in a SSF using an acetic acid free-hydrolysate evidenced the negative effect of acetic acid on the yeast-based xylitol production process. The combination of these green technologies and the optimization of the proposed strategy enhanced the overall xylitol production through the valorization of corn cob.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBaptista, S.L., Carvalho, L.C., Romaní, A., Domingues, L. (2020). Development of a sustainable bioprocess based on green technologies for xylitol production from corn cob. Industrial Crops and Products, 156:112867pt_PT
dc.identifier.doi10.1016/j.indcrop.2020.112867pt_PT
dc.identifier.issn0926-6690
dc.identifier.urihttp://hdl.handle.net/10400.14/31618
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation0688_BIOVINO_6_Ept_PT
dc.relationCentre of Biological Engineering of the University of Minho
dc.subjectAutohydrolysispt_PT
dc.subjectAdded-value chemicalpt_PT
dc.subjectCorn cob whole-slurrypt_PT
dc.subjectIndustrial Saccharomyces cerevisiaept_PT
dc.subjectSimultaneous saccharification and fermentationpt_PT
dc.subjectXylitolpt_PT
dc.titleDevelopment of a sustainable bioprocess based on green technologies for xylitol production from corn cobpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.citation.titleIndustrial Crops and Productspt_PT
oaire.citation.volume156pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBaptista
person.familyNameRomaní
person.givenNameSara
person.givenNameAloia
person.identifier.ciencia-id8A12-6AA4-F2D7
person.identifier.ciencia-idD810-4769-760E
person.identifier.orcid0000-0002-7921-3628
person.identifier.orcid0000-0001-6062-5529
person.identifier.ridO-8272-2015
person.identifier.scopus-author-id57202980274
person.identifier.scopus-author-id22942377000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication8bac013d-90ce-417e-b3cd-26a62830a681
relation.isAuthorOfPublication38ee4da4-5646-4830-b6df-ad8394eea780
relation.isAuthorOfPublication.latestForDiscovery8bac013d-90ce-417e-b3cd-26a62830a681
relation.isProjectOfPublication664dbf58-4f12-4cc0-8633-1c74a4848872
relation.isProjectOfPublication.latestForDiscovery664dbf58-4f12-4cc0-8633-1c74a4848872

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