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Chitosan-based silver nanoparticles: a study of the antibacterial, antileishmanial and cytotoxic effects

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dc.contributor.authorLima, Douglas dos Santos
dc.contributor.authorGullon, Beatriz
dc.contributor.authorCardelle-Cobas, Alejandra
dc.contributor.authorBrito, Lucas M.
dc.contributor.authorRodrigues, Klinger A. F.
dc.contributor.authorQuelemes, Patrick V.
dc.contributor.authorRamos-Jesus, Joilson
dc.contributor.authorArcanjo, Daniel D. R.
dc.contributor.authorPlácido, Alexandra
dc.contributor.authorBatziou, Krystallenia
dc.contributor.authorQuaresma, Pedro
dc.contributor.authorEaton, Peter
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorCarvalho, Fernando Aecio
dc.contributor.authorSilva, Durcilene Alves da
dc.contributor.authorPintado, M. E.
dc.contributor.authorLeite, José Roberto de Sá
dc.date.accessioned2017-01-04T15:48:09Z
dc.date.available2017-01-04T15:48:09Z
dc.date.issued2017
dc.description.abstractSilver nanoparticles have been studied as an alternative for treatment of microbial infections and leishmaniasis, without promoting induction of microbial or parasite resistance. In this study, chitosan-based silver nanoparticles were synthesized from silver nitrate (AgNO3), sodium borohydride as a reducing agent, and the biopolymer chitosan as a capping agent. The chitosan-based silver nanoparticles were characterized by ultraviolet-visible, Fourier transform infrared, dynamic light scattering, zeta potential, atomic force microscopy, and transmission electron microscope. The antibacterial assay was performed by determination of the minimum inhibitory concentration. The antileishmanial and the cytotoxic effects induced by AgNO3, chitosan, and chitosan-based silver nanoparticles were analyzed by resazurin and MTT colorimetric assays, respectively. AgNO3, chitosan, and chitosan-based silver nanoparticles induced a marked activity against all bacterial strains and promastigote forms of Leishmania amazonensis at minimum inhibitory concentrations ranging from 1.69 to 3.38 μg Ag/mL. Interestingly, the chitosan-based silver nanoparticles presented less cytotoxicity than the AgNO3 alone and were more active against L. amazonensis than solely chitosan. Furthermore, the cytotoxic concentrations (CC50) of both chitosan and chitosan-based silver nanoparticles against macrophages were significantly higher than the IC50 against promastigotes. Thus, the chitosan-based silver nanoparticles represent a promising alternative for the treatment of microbial infections and leishmaniasis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLIMA, Douglas dos Santos; GULLON, Beatriz; CARDELLE-COBAS, Alejandra; BRITO, Lucas M.; RODRIGUES, Klinger A.F.; QUELEMES, Patrick V.; RAMOS-JESUS, Joilson; ARCANJO, Daniel D.R.;PLÁCIDO, Alexandra; BATZIOU, Krystallenia; QUARESMA, Pedro; EATON, Peter; DELERUE-MATOS, Cristina; CARVALHO, Fernando Aecio A.; SILVA, Durcilene Alves da; PINTADO, M. E.; LEITE, José Roberto de Sá - Chitosan-based silver nanoparticles: A study of the antibacterial, antileishmanial and cytotoxic effects. Journal of Bioactive and Compatible Polymers. ISSN 1530-8030. (2016), p. 1–14pt_PT
dc.identifier.doi10.1177/0883911516681329pt_PT
dc.identifier.eid85024486348
dc.identifier.issn0883-9115
dc.identifier.urihttp://hdl.handle.net/10400.14/21192
dc.identifier.wos000405508600004
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSAGE Publications
dc.relationPOCI/01/0145/FERDER/007728pt_PT
dc.relationStrategic Project - LA 6 - 2011-2012
dc.subjectAntibacterial activitypt_PT
dc.subjectAntileishmanial activitypt_PT
dc.subjectChitosanpt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectLeishmaniapt_PT
dc.subjectMTTpt_PT
dc.subjectResazurinpt_PT
dc.subjectSilver nanoparticlespt_PT
dc.titleChitosan-based silver nanoparticles: a study of the antibacterial, antileishmanial and cytotoxic effectspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 6 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FEQB%2FLA0006%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97995%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04378%2F2013/PT
oaire.citation.endPage410
oaire.citation.issue4
oaire.citation.startPage397
oaire.citation.titleJournal of Bioactive and Compatible Polymers
oaire.citation.volume32
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStreamSFRH
oaire.fundingStream5876
person.familyNameGullón Estévez
person.familyNameCardelle Cobas
person.familyNameRodrigues
person.familyNameVeras Quelemes
person.familyNameArcanjo
person.familyNamePlácido
person.familyNameEaton
person.familyNameDelerue-Matos
person.familyNameCarvalho
person.familyNameSilva
person.familyNamePintado
person.familyNameLeite
person.givenNameBeatriz
person.givenNameAlejandra
person.givenNameKlinger Antonio da Franca
person.givenNamePatrick
person.givenNameDaniel
person.givenNameAlexandra
person.givenNamePeter
person.givenNameCristina
person.givenNameFernando
person.givenNameDurcilene
person.givenNameMaria Manuela
person.givenNameJosé Roberto
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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