Repository logo
 
Publication

Degree of contamination of gutta-percha points by Staphylococcus aureus (MRSA/MSSA) strains

2024-08-06Journal article Open access
Simple item page
dc.contributor.authorTeles, Ana Moura
dc.contributor.authorPina, Cristina
dc.contributor.authorCardoso, Inês Lopes
dc.contributor.authorTramontana, Antea
dc.contributor.authorCardoso, Miguel
dc.contributor.authorDuarte, Ana Sofia
dc.contributor.authorBartolomeu, Maria
dc.contributor.authorNoites, Rita
dc.date.accessioned2024-09-18T14:50:07Z
dc.date.available2024-09-18T14:50:07Z
dc.date.issued2024-08-06
dc.description.abstractMethicillin-resistant Staphylococcus aureus (MRSA) is considered one of the most harmful bacteria to human health. Dentistry, like all healthcare disciplines, places great emphasis on preventing scenarios that may result in cross-infection. Although various tested and already used materials are suitable for filling the root canal system, Gutta-Percha (GP) remains the preferred and widely accepted gold standard. Objective: We performed an in vitro analysis of the contamination of GP points, regarding the strains of Methicillin-resistant (MRSA) and Methicillin-sensitive (MSSA) Staphylococcus aureus, using classical microbiology methods and molecular biology techniques. Methods: Gutta-Percha points of two different brands from opened packages (already in use for 1 month) were collected for analysis. The assessment involved incubating the GP points in Brain Heart Infusion (BHI) medium to detect microbial growth. Growing microorganisms were plated on a selective and differential chromogenic medium for MRSA/MSSA strains, and the identification of isolates was confirmed by Polymerase Chain Reaction (PCR). In the case of microbial growth, the GP point was submitted to a disinfection protocol. Results: From the 315 collected GP points, only 6 (1.9%) resulted in being positive for microbial growth. After confirmation by PCR, only one sample of the six GP points was contaminated by MRSA, and the remaining five were MSSA-contaminated. The disinfection protocol was effective in all contaminated GP points. Conclusions: The Gutta-Percha points from opened pre-sterilized packages showed a very low degree of contamination by MRSA/MSSA. However, the detection of MSSA and MRSA strains raises concerns about potential contamination in dental clinic environments, and this risk cannot be considered negligible.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms25168566pt_PT
dc.identifier.eid85202629370
dc.identifier.issn1661-6596
dc.identifier.pmcPMC11354985
dc.identifier.pmid39201252
dc.identifier.urihttp://hdl.handle.net/10400.14/46589
dc.identifier.wos001307131500001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectContaminationpt_PT
dc.subjectEndodonticspt_PT
dc.subjectGutta-Perchapt_PT
dc.subjectMRSA/MSSApt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.titleDegree of contamination of gutta-percha points by Staphylococcus aureus (MRSA/MSSA) strainspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue16pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume25pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
105166605.pdf
Size:
851.56 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.44 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIIS - Contribuições em Revistas Científicas / Contribution to Journals