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Process optimization of microwave-assisted extraction of bioactive molecules from avocado seeds

2020Journal article Restricted access
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dc.contributor.authorAraújo, Rafael G.
dc.contributor.authorRodriguez-Jasso, Rosa M.
dc.contributor.authorRuiz, Héctor A.
dc.contributor.authorGovea-Salas, Mayela
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorAguilar, Cristóbal N.
dc.date.accessioned2020-06-15T08:34:00Z
dc.date.available2020-06-15T08:34:00Z
dc.date.issued2020
dc.description.abstractAvocado seeds are an agroindustrial residue produced largely by avocado processing industries, which is accumulated in high quantities because it has low reuse due to its poor revalorization. The optimization of Microwave-Assisted Extraction (MAE) of bioactive compounds with high antioxidant capacity from avocado seeds were studied in the present work for the first time. Applying two experimental designs, using acetone 70 % and ethanol, the optimal conditions of extraction were found: 72.18 °C for 19.01 min and 71.64 °C for 14.69 min with 58.51 % of ethanol, respectively, to obtain extracts with highest antioxidant activity. The optimized extracts obtained with acetone and ethanol showed a high polyphenolic content (307.09 ± 14.16 and 254.40 ± 16.36 mg GAE/g extract) and high antioxidant activity measured by DPPH (266.56 ± 2.76 and 221.69 ± 20.12 mg ET/g extract), ABTS (607.28 ± 4.71 and 516.34 ± 11.81 mg ET/g extract) and ORAC (475.55 ± 47.82 and 495.25 ± 14.52 mg ET/g extract), proving MAE as a better technique than those previously reported for avocado seeds. Fiber residues obtained after MAE showed bonded phenolics compounds with relevant antioxidant activity, those obtained with ethanol. HPLC-ESI-MS analysis showed the presence of 20 different identified compounds, including phenolic acids, procyanidins dimer B and trimer A and B in different isomers forms, catechin, epicatechin and perseitol. MAE is a green, energy efficient and rapid tool for extractions of bioactive components from avocado seedswithout affecting their antioxidant activities representing an attractive alternative protocol in traditional herbal industry and medicine.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.citationAraújo, R.G., Rodriguez-Jasso, R.M., Ruiz, H.A., Govea-Salas, M., Pintado, M.E., Aguilar, C.N. (2020). Process optimization of microwave-assisted extraction of bioactive molecules from avocado seeds. Industrial Crops and Products, 154, art. n.º 112623pt_PT
dc.identifier.doi10.1016/j.indcrop.2020.112623pt_PT
dc.identifier.eid85086008770
dc.identifier.issn0926-6690
dc.identifier.urihttp://hdl.handle.net/10400.14/30710
dc.identifier.wos000554526900017
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCONACYT-PEI 251902pt_PT
dc.subjectMicrowave-assisted extractionpt_PT
dc.subjectAgroindustrial residuespt_PT
dc.subjectAntioxidant capacitypt_PT
dc.subjectBiomass valorizationpt_PT
dc.titleProcess optimization of microwave-assisted extraction of bioactive molecules from avocado seedspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.citation.titleIndustrial Crops and Productspt_PT
oaire.citation.volume154pt_PT
oaire.fundingStream5876
person.familyNamePintado
person.givenNameMaria Manuela
person.identifier456608
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoveryba387c7d-27c9-4016-895c-b35597e91ebc
relation.isProjectOfPublication3fe5970e-de39-42f7-a29d-18521b591a09
relation.isProjectOfPublication.latestForDiscovery3fe5970e-de39-42f7-a29d-18521b591a09

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