Publicação
Exploring the potential of mealworm chitosan for hemodialysis applications
| dc.contributor.author | Martingo, Maria | |
| dc.contributor.author | Baptista-Silva, Sara | |
| dc.contributor.author | Mesquita, Raquel | |
| dc.contributor.author | Ferreira, João Paulo | |
| dc.contributor.author | Borges, Sandra | |
| dc.contributor.author | Pintado, Manuela | |
| dc.date.accessioned | 2025-04-15T17:16:29Z | |
| dc.date.available | 2025-04-15T17:16:29Z | |
| dc.date.issued | 2025-06 | |
| dc.description.abstract | This study introduces a sustainable and efficient alternative to traditional chitosan sources derived from crustaceans, exploring the extraction and application of insect-derived chitosan from Tenebrio molitor for hemodialysis (HD) membranes design. Efficient extraction and deacetylation methods were tested and developed between 6 h and 12 h to obtain chitosan. Chitin was isolated from T. molitor through deproteinization and demineralization, with yields of approximately 5 % (w/w). Chitosan was obtained from the extracted chitin resulting in yields of between 65.0 and 79.3 (w/w). Characterization using FTIR confirmed structural similarities with commercial chitosan and degrees of deacetylation in the 73–75 % range. The bioactive properties of chitosan obtained from T. molitor, including antimicrobial and antioxidant activities, were evaluated. All the microorganisms tested were inhibited, exhibiting minimum lethal concentrations between 2 and 8 mg/mL. In addition, chitosan showed antioxidant activity in the range of 60–65 μmol Trolox equivalent/g, suggesting its viability for various medical applications. This study additionally allowed the design of sustainable hybrid chitosan membrane (CH-M) tailored for HD applications. The permeation characteristics of CH-M for urea and albumin were studied in vitro to assess their suitability as HD membranes. Urea was permeable to values of over 70 % and albumin was retained. Also, cytotoxicity assays against L929 fibroblast cells demonstrated that the CH-M samples exhibit low metabolic inhibition (around 15 %) The application of CH-M in HD represents a significant advance, offering the potential for enhanced therapeutic outcomes for chronic kidney disease (CKD). | eng |
| dc.identifier.doi | 10.1016/j.scp.2025.102013 | |
| dc.identifier.eid | 105001872230 | |
| dc.identifier.issn | 2352-5541 | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/52984 | |
| dc.identifier.wos | 001468430500001 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Bio-based membrane | |
| dc.subject | Chitosan | |
| dc.subject | Hemodialysis treatment | |
| dc.subject | Tenebrio molitor | |
| dc.title | Exploring the potential of mealworm chitosan for hemodialysis applications | eng |
| dc.type | research article | |
| dspace.entity.type | Publication | |
| oaire.citation.title | Sustainable Chemistry and Pharmacy | |
| oaire.citation.volume | 45 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |