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Evaluating prebiotic potential

dc.contributor.authorAraújo-Rodrigues, Helena
dc.contributor.authorSalsinha, Ana Sofia
dc.contributor.authorRelvas, João B.
dc.contributor.authorTavaria, Freni K.
dc.contributor.authorPintado, Manuela
dc.date.accessioned2026-01-22T17:32:24Z
dc.date.available2026-01-22T17:32:24Z
dc.date.issued2025-10-28
dc.description.abstractMushrooms represent a promising source of bioactive macromolecules with numerous biological properties, including prebiotic potential. Although most studies focus on polysaccharide extracts, emerging evidence suggests that diverse mushroom macromolecules, such as proteins, peptides, fatty acids, and polyphenolic compounds, may also exert prebiotic effects. This chapter comprehensively explores the methodological approaches for the evaluation of the prebiotic properties of edible fungi, emphasizing the gastrointestinal simulation and gut microbiota modulation approaches. The standardized INFOGEST protocol simulates human gastrointestinal conditions, helping to understand the bioaccessibility of mushroom-derived compounds. Moreover, in vitro intestinal permeability assays are used to determine the colon-available fraction. Human fecal fermentation assays are frequently employed to assess mushrooms’ prebiotic potential and its impact on gut microbiota modulation. These studies typically monitor key parameters, including their impact on bacterial population dynamics, production of SCFAs, and other relevant metabolic variations (e.g., amino acid metabolism). By integrating in vitro digestion and fermentation models with advanced molecular biology, microbiological, and chemical techniques, this chapter provides a systematic outline for evaluating the prebiotic potential of mushroom compounds and understanding their interaction with gut microbiota composition and function.eng
dc.identifier.citationAraújo-Rodrigues, H., Salsinha, A. S., Relvas, J. B., & Tavaria, F. K. et al. (2025). Evaluating prebiotic potential. In P. Navarro Simarro (Ed.), Fungi (pp. 145-171). (Methods and Protocols in Food Science). Humana Press. https://doi.org/10.1007/978-1-0716-4989-3_12
dc.identifier.doi10.1007/978-1-0716-4989-3_12
dc.identifier.isbn9781071649893
dc.identifier.isbn9781071649886
dc.identifier.isbn9781071649916
dc.identifier.other4edc926f-f18d-4f59-a507-f715c425845b
dc.identifier.urihttp://hdl.handle.net/10400.14/56637
dc.language.isoeng
dc.peerreviewedyes
dc.publisherHumana Press
dc.rights.uriN/A
dc.subjectAmino acid production
dc.subjectAmmonia production
dc.subjectBacterial dynamics
dc.subjectGastrointestinal simulation
dc.subjectGut microbiota modulation
dc.subjectPrebiotics
dc.subjectShort-chain fatty acids
dc.titleEvaluating prebiotic potentialeng
dc.typebook part
dspace.entity.typePublication
oaire.citation.endPage171
oaire.citation.startPage145
oaire.versionhttp://purl.org/coar/version/c_ab4af688f83e57aa

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