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Enantioselective Determination of Fluoxetine and Norfluoxetine in Wastewater

2013Conference object Open access
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dc.contributor.authorRibeiro, Ana R.
dc.contributor.authorMaia, Alexandra S.
dc.contributor.authorMoreira, Irina S.
dc.contributor.authorAfonso, Carlos
dc.contributor.authorCastro, Paula M.L.
dc.contributor.authorTiritan, Maria E.
dc.date.accessioned2013-11-14T16:28:00Z
dc.date.available2013-11-14T16:28:00Z
dc.date.issued2013
dc.description.abstractMicrobial degradation of chiral compounds during wastewater treatment processes can be enantioselective and needs chiral analytical methodology to discriminate the biodegradation of both enantiomers. An enantioselective HPLC-FD method was developed and validated to monitor the degradation of fluoxetine (FLX) enantiomers by wastewater and the possible formation of its metabolite norfluoxetine (NFLX). The Solid Phase Extraction (SPE) of 50 mL of wastewater samples on 500 mg Oasis MCX cartridges was followed by the HPLC analysis using a Chirobiotic V chiral stationary phase under reversed mode. The developed method wasvalidated within the wastewater effluent used in microcosms laboratory assays. The chiral SPE-HPLC-FD method demonstrated to be selective, linear, sensitive, accurate and precise to quantify the enantiomers of FLX and of its metabolites NFLX in wastewaters. The limits of detection (0.8-2.0 ng mL -1 ) and quantification (2.0 – 4.0 ng mL -1 ) were adequate to monitoring the degradation assays at environmental level. The method proved to be robust to follow the biodegradation assays using real wastewater samplesspiked with FLX, during 46 days. To the best of our knowledge, this is the first reportof simultaneous separation of FLX and NFLX enantiomers using a Chirobiotic V and the application of the validated method to the enantioselective degradation by wastewaterpor
dc.identifier.citationRIBEIRO, Ana R. [et al.] - Enantioselective Determination of Fluoxetine and Norfluoxetine in Wastewater. In 39th International Symposium on High Performance Liquid Phase Separations and Related Techniques, Amsterdam, The Netherlands, 16-20 June 2013 – In HPLC 2013 - 39th International Symposium on High Performance Liquid Phase Separations and Related Techniques: Book of Abstracts, (2013) p.290por
dc.identifier.urihttp://hdl.handle.net/10400.14/13241
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectBiodegradationpor
dc.subjectChiralpor
dc.subjectPharmaceuticalspor
dc.titleEnantioselective Determination of Fluoxetine and Norfluoxetine in Wastewaterpor
dc.typeconference object
dspace.entity.typePublication
person.familyNameMaia
person.familyNameMoreira
person.familyNameAfonso
person.familyNameCastro
person.familyNameTiritan
person.givenNameAlexandra S.
person.givenNameIrina Susana Sousa
person.givenNameCarlos
person.givenNamePaula
person.givenNameMaria Elizabeth
person.identifierK-5841-2014
person.identifier2013444
person.identifier1310409
person.identifier.ciencia-id6A15-F0A3-AEF2
person.identifier.ciencia-id161E-A350-7FDE
person.identifier.ciencia-id6A10-3565-6158
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.ciencia-idD915-BFC0-FB3A
person.identifier.orcid0000-0002-4167-383X
person.identifier.orcid0000-0001-6516-0994
person.identifier.orcid0000-0002-5731-5789
person.identifier.orcid0000-0001-8841-6606
person.identifier.orcid0000-0003-3320-730X
person.identifier.ridK-5841-2014
person.identifier.ridI-5989-2015
person.identifier.ridM-8241-2013
person.identifier.ridK-9492-2014
person.identifier.scopus-author-id55332349300
person.identifier.scopus-author-id36798094100
person.identifier.scopus-author-id36145043600
person.identifier.scopus-author-id7102781782
person.identifier.scopus-author-id6602890981
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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