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Adhesion of Candida albicans and Streptococcus mutans to the surface of a 3D-printed resin used for the fabrication of prosthetic bases

2026-07-14Artigo de investigação Acesso aberto
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dc.contributor.authorQuezada, Margarida Martins
dc.contributor.authorFonseca, Patrícia
dc.contributor.authorGomes, Ana T. P. C.
dc.contributor.authorDuarte, Ana S.
dc.contributor.authorFernandes, Hugo R.
dc.contributor.authorCorreia, André
dc.contributor.authorSalgado, Helena
dc.date.accessioned2026-08-06T15:24:22Z
dc.date.available2026-08-06T15:24:22Z
dc.date.issued2026-07-14
dc.description.abstractBackground: Surface roughness and microbial adhesion are key determinants of the biological performance of prosthetic base resins. This study evaluated Candida albicans (ATCC 11225) and Streptococcus mutans (DSM 20523) adhesion to a conventional heat-polymerized acrylic resin (Probase Hot) and a 3D-printed resin (V-Print dentbase) fabricated at 0° and 90° build orientations. Methods: Fifteen disc-shaped specimens (10 mm × 2 mm) were polished using a standardized protocol and surface roughness was characterized by contact profilometry (Ra). After UV sterilization, specimens were incubated for 24 h with standardized suspensions of C. albicans or S. mutans. Microbial adhesion was assessed qualitatively by scanning electron microscopy (SEM). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test after confirmation of normality (Shapiro–Wilk) and homogeneity of variances (Levene). Statistical significance was set at p < 0.05, and Cohen’s d was calculated to estimate effect size. Results: Surface roughness differed significantly among fabrication conditions (p = 0.017), with the conventional heat-polymerized resin showing the highest Ra values and the 3D-printed resin fabricated at 90° presenting the lowest roughness. SEM observations suggested a similar adhesion pattern for both C. albicans and S. mutans, with greater apparent microbial presence on 90°-printed specimens, followed by the conventional heat-polymerized resin, whereas 0°-printed specimens showed lower apparent adhesion in the analyzed fields. Conclusions: These findings suggest that printing orientation may influence surface-associated microbial adhesion patterns in 3D-printed prosthetic resins; however, further studies with larger sample sizes, orientation-specific surface characterization, and mono- and multispecies biofilm models are warranted to confirm these preliminary findings.eng
dc.identifier.doi10.3390/app16147047
dc.identifier.eid105045964499
dc.identifier.otherdd652255-2bf7-4933-90af-cb084f9b0af1
dc.identifier.urihttp://hdl.handle.net/10400.14/59028
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject3Deng
dc.subjectCandida albicanseng
dc.subjectDenture base resineng
dc.subjectMicrobial adhesioneng
dc.subjectPMMAeng
dc.subjectPrinting orientationeng
dc.subjectScanning electron microscopyeng
dc.subjectStreptococcus mutanseng
dc.subjectSurface roughnesseng
dc.titleAdhesion of Candida albicans and Streptococcus mutans to the surface of a 3D-printed resin used for the fabrication of prosthetic bases
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.issue14
oaire.citation.volume16
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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