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Effect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. bulgaricus to stress conditions during spray-drying

2005Journal article Open access
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dc.contributor.authorSilva, J.
dc.contributor.authorCarvalho, A. S.
dc.contributor.authorFerreira, R.
dc.contributor.authorVitorino, R.
dc.contributor.authorAmado, F.
dc.contributor.authorDomingues, P.
dc.contributor.authorTeixeira, P.
dc.contributor.authorGibbs, P. A.
dc.date.accessioned2010-10-12T10:15:45Z
dc.date.available2010-10-12T10:15:45Z
dc.date.issued2005
dc.description.abstractAims: The aim of this study was to optimize survival of Lactobacillus delbrueckii subsp. bulgaricus during spraydrying and subsequent storage through optimizing the pH of growth conditions. Methods and Results: Cell concentrates previously grown without or with pH controlled were spray-dried and stored at 20ºC and heat treated at 57ºC. Cells grown under noncontrolled pH were more resistant to both drying and heating than cells grown under controlled pH but no significant differences were observed during storage. The intracellular proteins profile of cells grown under both conditions was studied by two-dimensional SDS-polyacrylamide gel electrophoresis. Eight proteins were identified using automated mass spectrometry (MS) and tandem mass spectrometry (MS/MS) data acquisition. Of the identified proteins, only cochaperonin GroES corresponded to a known heat shock protein (HSP). The other proteins identified are proteins involved in glycolysis. For cells grown under noncontrolled pH the expression of the Hsp70, GroES and GroEL, measured by Western blotting, was enhanced. Conclusions: The higher resistance of cells grown under noncontrolled pH correlates with the enhanced production of heat shock proteins. Significance and Impact of the Study: Growth of L. bulgaricus under controlled pH (commonly used by the starter cultures production industry) results in cells more sensitive to stresses frequently encountered by the cells during starter cultures preparation/storage/utilization.por
dc.identifier.citationSILVA, J...[et al.] - Effect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. bulgaricus to stress conditions during spray-drying. Journal of Applied Microbiology. ISSN 1364-5072. Vol. 98, n.º 3 (2005), p. 775–782por
dc.identifier.doi10.1111/j.1365-2672.2004.02516.x
dc.identifier.urihttp://hdl.handle.net/10400.14/2857
dc.language.isoengpor
dc.publisherWiley Blackwellpor
dc.subjectHeat shock proteinspor
dc.subjectLactobacillus bulgaricuspor
dc.subjectpH growth conditionspor
dc.subjectSpray-dryingpor
dc.subjectStoragepor
dc.subjectThermotolerancepor
dc.titleEffect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. bulgaricus to stress conditions during spray-dryingpor
dc.typejournal article
dspace.entity.typePublication
person.familyNameSilva
person.familyNameTeixeira
person.familyNameGibbs
person.givenNameJoana
person.givenNamePaula
person.givenNamePaul
person.identifier.ciencia-idB415-743D-D9F9
person.identifier.orcid0000-0003-3977-7418
person.identifier.orcid0000-0002-6296-5137
person.identifier.orcid0000-0002-6580-4363
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id7403023130
person.identifier.scopus-author-id7005895206
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication42069f79-2050-47f2-a42b-fab5c301e9a3
relation.isAuthorOfPublicationb918d4a2-b9f9-4c56-8318-111625e4d58b
relation.isAuthorOfPublication218d85c8-40f4-42c0-9a47-4e8ad9f72a96
relation.isAuthorOfPublication.latestForDiscoveryb918d4a2-b9f9-4c56-8318-111625e4d58b

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