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Industrial wastewater streams are difficult to manage due to their complexity, often containing several organic and inorganic compounds, such as toxic/recalcitrant organics, ammonium and inorganic salts. Aerobic granular sludge (AGS) technology has been widely investigated for the treatment of urban and industrial wastewater and its implementation at full-scale is growing worldwide. AGS is a special type of biofilm, formed via self-aggregation of microorganisms in an extracellular polymeric substances (EPS) matrix. EPS produced by microorganisms are crucial not only for granules formation and stability but also to protect cells against harsh conditions in the living environment which often occur in industrial wastewater streams.In this study, the combined effect of different anthropogenic substances on the EPS production by AGS was evaluated.