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Lettuce and fruits as a source of multidrug resistant Acinetobacter spp.

2017Journal article Restricted access
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dc.contributor.authorCarvalheira, Ana
dc.contributor.authorSilva, Joana
dc.contributor.authorTeixeira, Paula
dc.date.accessioned2017-12-18T15:47:27Z
dc.date.available2017-12-18T15:47:27Z
dc.date.issued2017
dc.description.abstractThe role of ready-to-eat products as a reservoir of pathogenic species of Acinetobacter remains unclear. The objective of the present study was to evaluate the presence of Acinetobacter species in lettuces and fruits marketed in Portugal, and their susceptibility to antimicrobials. Acinetobacter spp. were isolated from 77.9% of the samples and these microorganisms were also found as endophytes (i.e. present within the plant tissue) in 12 of 20 samples of lettuces analysed. Among 253 isolates that were identified as belonging to this genus, 181 presented different PFGE profiles, representing different strains. Based on the analysis of the partial sequence of rpoB, 175 strains were identified as members of eighteen distinct species and the remaining six strains may represent five new candidate species since their rpoB sequence similarities with type strains were less than 95%. Acinetobacter calcoaceticus and Acinetobacter johnsonii were the most common species, both with the frequency of 26.5%; and 11% of the strains belong to the Acinetobacter baumannii group (i.e. A. baumannii, Acinetobacter pittii, Acinetobacter seifertii and Acinetobacter nosocomialis), which is most frequently associated with nosocomial infections. Overall, the strains were least susceptible to piperacillin (80.1%), piperacillin-tazobactam (64.1%), ceftazidime (43.1%), ciprofloxacin (16.6%), trimethoprim-sulfamethoxazole (14.9%), imipenem (14.4%) and colistin (13.3%). The most active antimicrobials were minocycline and tetracycline, with 0.6% and 3.9% of strains resistant, respectively. About 29.8% of the strains were classified as multidrug-resistant (MDR), 4.4% as extensively drug-resistant (XDR) and the prevalence of MDR strains within the A. baumannii group (25%) was similar to other species (30.4%). The presence of clinically important species as well as MDR strains in lettuces and fruits may be a threat to public health considering that they may transmit these pathogens to environments such as the community and hospital settings.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCarvalheira, A., Silva, J., Teixeira, P. (2017). Lettuce and fruits as a source of multidrug resistant acinetobacter spp. Food Microbiology, 64, 119-125pt_PT
dc.identifier.doi10.1016/j.fm.2016.12.005pt_PT
dc.identifier.eid85008374961
dc.identifier.issn0740-0020
dc.identifier.pmid28213015
dc.identifier.urihttp://hdl.handle.net/10400.14/23707
dc.identifier.wos000395842500015
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationACINETOBACTER AND PUBLIC HEALTH: RISKS POSED BY STRAINS ISOLATED FROM COMMUNITY AND HOSPITAL
dc.relationISOLATION, CHARACTERIZATION, VIRULENCE FACTORS AND ANTIBIOTIC RESISTANCE AMONG CAMPYLOBACTER SPP. ISOLATED FROM FOOD AND CLINICAL CASES
dc.subjectAcinetobacterpt_PT
dc.subjectAcinetobacter baumanniipt_PT
dc.subjectAnti-Bacterial Agentspt_PT
dc.subjectBacterial Proteinspt_PT
dc.subjectElectrophoresis, Gel, Pulsed-Fieldpt_PT
dc.subjectEndophytespt_PT
dc.subjectFood Microbiologypt_PT
dc.subjectFruitpt_PT
dc.subjectLettucept_PT
dc.subjectMicrobial Sensitivity Testspt_PT
dc.subjectMinocyclinept_PT
dc.subjectPortugalpt_PT
dc.subjectTetracyclinept_PT
dc.subjectDrug Resistance, Multiple, Bacterialpt_PT
dc.titleLettuce and fruits as a source of multidrug resistant Acinetobacter spp.pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleACINETOBACTER AND PUBLIC HEALTH: RISKS POSED BY STRAINS ISOLATED FROM COMMUNITY AND HOSPITAL
oaire.awardTitleISOLATION, CHARACTERIZATION, VIRULENCE FACTORS AND ANTIBIOTIC RESISTANCE AMONG CAMPYLOBACTER SPP. ISOLATED FROM FOOD AND CLINICAL CASES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F72951%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F35392%2F2007/PT
oaire.citation.endPage125pt_PT
oaire.citation.startPage119pt_PT
oaire.citation.titleFood Microbiologypt_PT
oaire.citation.volume64pt_PT
oaire.fundingStream5876
person.familyNameSilva
person.familyNameTeixeira
person.givenNameJoana
person.givenNamePaula
person.identifier.ciencia-idB415-743D-D9F9
person.identifier.orcid0000-0003-3977-7418
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id7403023130
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationb918d4a2-b9f9-4c56-8318-111625e4d58b
relation.isAuthorOfPublication.latestForDiscoveryb918d4a2-b9f9-4c56-8318-111625e4d58b
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