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Transcriptional and biochemical regulation of internal browning disorder in 'Rocha' pear as affected by O-2 and CO2 concentrations

2017Journal article Restricted access
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dc.contributor.authorDeuchande, Teresa
dc.contributor.authorCarvalho, Susana M. P.
dc.contributor.authorGiné-Bordonaba, Jordi
dc.contributor.authorVasconcelos, Marta W.
dc.contributor.authorLarrigaudière, Christian
dc.date.accessioned2017-12-18T18:41:38Z
dc.date.available2017-12-18T18:41:38Z
dc.date.issued2017
dc.description.abstractThe biochemical basis of internal browning disorders (IBD) in ‘Rocha’ pear has been linked to alterations in the antioxidant and fermentative metabolism. In order to further elucidate the involvement of these metabolic pathways on IBD development, we investigated their regulation at a metabolic and transcriptional level in pears that were stored under four distinct conditions: i) cold air (−0.5 °C); ii) standard controlled atmosphere (CA; 2 kPa O2 + 0.5 kPa CO2); iii) high CO2 (2 kPa O2 + 10 kPa CO2), and iv) high CO2 + low O2 (O2-switch: 60 d under high CO2 + 80 d under high CO2 and 1 kPa O2). Only the fruit stored under high CO2 were affected by IBD. The faster depletion of ascorbate in high CO2-stored fruit was associated with the down-regulation of glutathione reductase (PcGR) and ascorbate peroxidase (PcAPX) as well as a down-regulation of monodehydroascorbate reductase (PcMDHAR). The O2-switched fruit had the highest IBD incidence. Increased incidence in this sample was associated to a sharp increase in fermentation immediately after transfer to lower O2 atmosphere suggesting a synergistic effect between high CO2 and low O2 concentrations in IBD development. Overall, our results suggest that biochemical and transcriptional regulation of the antioxidant system affects IBD occurrence in ‘Rocha’ pear, and that fermentation, resulting from fruit exposure to low O2 concentrations, may further promote the browning process.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDeuchande, T., Carvalho, S. M. P., Gine-Bordonaba, J., Vasconcelos, M. W., & Larrigaudiere, C. (2017). Transcriptional and biochemical regulation of internal browning disorder in 'rocha' pear as affected by O-2 and CO2 concentrations. Postharvest Biology and Technology, 132, 15-22pt_PT
dc.identifier.doi10.1016/j.postharvbio.2017.05.011pt_PT
dc.identifier.eid85019981938
dc.identifier.eissn1873-2356
dc.identifier.issn0925-5214
dc.identifier.urihttp://hdl.handle.net/10400.14/23716
dc.identifier.wos000406983900003
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.relationPOCI-01-0145-FEDER-006958pt_PT
dc.subjectAcetaldehydept_PT
dc.subjectAscorbic acidpt_PT
dc.subjectBrowningpt_PT
dc.subjectEthanolpt_PT
dc.subjectGene expressionpt_PT
dc.subjectOxidative stress
dc.titleTranscriptional and biochemical regulation of internal browning disorder in 'Rocha' pear as affected by O-2 and CO2 concentrationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F73216%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F04033%2F2013/PT
oaire.citation.endPage22pt_PT
oaire.citation.startPage15pt_PT
oaire.citation.titlePostharvest Biology and Technologypt_PT
oaire.citation.volume132pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameDeuchande
person.familyNameCarvalho
person.familyNameVasconcelos
person.givenNameTeresa
person.givenNameSusana M.P.
person.givenNameMarta
person.identifier194747
person.identifier74696
person.identifier.ciencia-id1C17-8474-DA4F
person.identifier.ciencia-id5E16-859A-37A4
person.identifier.orcid0000-0002-7017-882X
person.identifier.orcid0000-0001-7157-1079
person.identifier.orcid0000-0002-5110-7006
person.identifier.ridI-7184-2013
person.identifier.ridI-8166-2013
person.identifier.scopus-author-id56604286100
person.identifier.scopus-author-id7005619826
person.identifier.scopus-author-id14627797100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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