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Advisor(s)
Abstract(s)
Hydrogels of spatially controlled physicochemical properties are appealing platforms for tissue engineering and drug delivery.
In this study, core-shell silk fibroin (SF) hydrogels of spatially controlled conformation were developed. The core-shell structure
in the hydrogels was formed by means of soaking the preformed (enzymatically crosslinked) random coil SF hydrogels in methanol.
When increasing the methanol treatment time from 1 to 10 min, the thickness of the shell layer can be tuned from about
200 to about 850 μm as measured in wet status. After lyophilization of the rehydrated core-shell hydrogels, the shell layer
displayed compact morphology and the core layer presented porous structure, when observed by scanning electron microscopy.
The conformation of the hydrogels was evaluated by Fourier transform infrared spectroscopy in wet status. The results revealed
that the shell layer possessed dominant β-sheet conformation and the core layer maintained mainly random coil conformation.
Enzymatic degradation data showed that the shell layers presented superior stability to the core layer. The mechanical analysis
displayed that the compressive modulus of the core-shell hydrogels ranged from about 25 kPa to about 1.1 MPa by increasing the
immersion time in methanol. When incorporated with albumin, the core-shell SF hydrogels demonstrated slower and more controllable
release profiles compared with the non-treated hydrogel. These core-shell SF hydrogels of highly tuned properties are
useful systems as drug-delivery system and may be applied as cartilage substitute
Description
Keywords
Silk fibroin Core-shell hydrogel Spatially controlled conformation Drug delivery Beta-sheet Random coil Tissue engineering Regenerative medicine
Pedagogical Context
Citation
LE-PING, Yan; OLIVEIRA, Joaquim M., OLIVEIRA, Ana L.; REIS, Rui L. - Core-shell silk hydrogels with spatially tuned conformations as drug-delivery system. Journal of Tissue Engineering and Regenerative Medicine. ISSN 1932-7005. (2016), 10 p.
Publisher
Wiley