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Plant aqueous extracts: antioxidant capacity via haemolysis and bacteriophage P22 protection

dc.contributor.authorGião, Maria S.
dc.contributor.authorLeitão, Isabel
dc.contributor.authorPereira, Ana
dc.contributor.authorBorges, André B.
dc.contributor.authorGuedes, Catarina J.
dc.contributor.authorFernandes, João C.
dc.contributor.authorBelo, Luís
dc.contributor.authorSantos-Silva, Alice
dc.contributor.authorHogg, Tim A.
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2010-10-04T11:14:43Z
dc.date.available2010-10-04T11:14:43Z
dc.date.issued2010
dc.description.abstractThe bacteriophage P22/Salmonella Typhimurium system, as well as human erythrocytes have been used to assay for protection, against forced oxidation caused by hydrogen peroxide, brought about by several aqueous extracts of selected adventitious plants grown in Portugal. This study proved, for the first time, that the aforementioned bacteriophage-based system is a suitable method to assess the antioxidant activity of plant extracts; among the 12 plants tested, raspberry (Rubus idaeus), sage (Salvia sp.), savory (Satureja montana) and yarrow (Achillea millefolium) were found to effectively protect against oxidative damage caused by H2O2. Haemolysis was inhibited via pre-treatment with every plant extract tested, except heath at 0.1% (w/v). The two analytical methods produced different results – and for some plants, there was a dependence (either direct or inverse) of the quantitative protection effect on extract concentration, whereas for others no significant dependence was found at all. Savory yielded the most promising results, using either method. Therefore, the P22/Salmonella system can be used as a suitable in vivo assay, and human erythrocytes as a suitable in vitro assay to confirm (or not) the antioxidant capacity of plant extracts in biological matrices.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.foodcont.2009.08.014
dc.identifier.eid73549116726
dc.identifier.eissn1873-7129
dc.identifier.issn0956-7135
dc.identifier.urihttp://hdl.handle.net/10400.14/2656
dc.identifier.wos000274843400008
dc.language.isoengpor
dc.publisherElsevierpor
dc.relation13-05-04 - FDR - 00020
dc.relationANTIOXIDANTS IN HERBAL TEAS AS FUNCTIONAL INGREDIENTS: EXTRACTION, CHARACTERIZATION, BIOAVAILABILITY, ACTIVITY AND APPLICATIONS
dc.subjectMedicinal plantspor
dc.subjectIn vivo assayspor
dc.subjectSalmonella Typhimuriumpor
dc.subjectErythrocytespor
dc.titlePlant aqueous extracts: antioxidant capacity via haemolysis and bacteriophage P22 protectionpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleANTIOXIDANTS IN HERBAL TEAS AS FUNCTIONAL INGREDIENTS: EXTRACTION, CHARACTERIZATION, BIOAVAILABILITY, ACTIVITY AND APPLICATIONS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F19601%2F2004/PT
oaire.citation.endPage638
oaire.citation.issue5
oaire.citation.startPage633
oaire.citation.titleFood Control
oaire.citation.volume21
person.familyNameFernandes
person.familyNameBelo
person.familyNameSantos-Silva
person.familyNameHogg
person.familyNamePintado
person.familyNameMalcata
person.givenNameJoão
person.givenNameLuís
person.givenNameAlice
person.givenNameTim
person.givenNameMaria Manuela
person.givenNameFrancisco
person.identifier1368242
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person.identifier.orcid0000-0003-3073-1659
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person.identifier.ridJXY-0365-2024
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id35274726800
person.identifier.scopus-author-id6602879850
person.identifier.scopus-author-id6603836490
person.identifier.scopus-author-id7005603860
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id7102542478
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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