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Manufacture of bioactive peptide-rich concentrates from whey: characterization of pilot process

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dc.contributor.authorTavares, Tânia G.
dc.contributor.authorAmorim, Manuela
dc.contributor.authorGomes, David
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorPereira, Carlos D.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2013-01-28T15:45:14Z
dc.date.available2013-01-28T15:45:14Z
dc.date.issued2012
dc.descriptionResearch Notepor
dc.description.abstractThis work was focused on the manufacture, at pilot scale, of cow whey protein and peptide concentrates, using selective filtration techniques—associated with hydrolysis brought about by proteolytic enzymes from Cynara cardunculus aqueous extracts, using as (optimal) conditions an enzyme/substrate ratio of 1.6% v/v, a pH of 5.2, a temperature of 55 C and an incubation time of 7 h. The profiles of proteins and peptides were assessed by liquid chromatography and electrophoresis; ca. 87% of a-lactalbumin was hydrolyzed, but essentially no degradation of b-lactoglobulin (b-Lg) was observed. A bioactive peptide concentrate, its fraction below 3 kDa and a b-Lg-rich fraction were obtained as final products, containing ca. 73, 43 and 91% w/w protein (on a total solid mass basis). All these fractions were low in lactose and salt, and their microbial loads were reduced. Said fractions are high added-value products, so they can be used as nutritional and functional ingredients—thus yielding an economically viable alternative for upgrade of whey.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationTAVARES, Tânia G. ...[et al] - Manufacture of bioactive peptide-rich concentrates from whey: characterization of pilot process. Journal of Food Engineering. ISSN 0260-8774. Vol. 110, n.º 4 (2012), p. 547-552por
dc.identifier.doi10.1016/j.jfoodeng.2012.01.009
dc.identifier.eissn1873-5770
dc.identifier.issn0260-8774
dc.identifier.urihttp://hdl.handle.net/10400.14/10023
dc.identifier.wosWOS:000302046700006
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectProcess engineeringpor
dc.subjectWhey upgradepor
dc.subjectNovel peptide-rich productpor
dc.subjectAntihypertensive featurespor
dc.titleManufacture of bioactive peptide-rich concentrates from whey: characterization of pilot processpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F31604%2F2006/PT
oaire.citation.endPage552
oaire.citation.issue4
oaire.citation.startPage547
oaire.citation.titleJournal of Food Engineering
oaire.citation.volume110
oaire.fundingStreamSFRH
person.familyNameGranja Tavares
person.familyNameAmorim
person.familyNameGomes
person.familyNamePintado
person.familyNamePereira
person.familyNameMalcata
person.givenNameTânia Sofia
person.givenNameMaria Manuela
person.givenNameDavid Manuel Gama
person.givenNameMaria Manuela
person.givenNameCarlos
person.givenNameFrancisco
person.identifier456608
person.identifier.ciencia-idDB16-AFE8-27BD
person.identifier.ciencia-id791D-E9BA-D432
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.ciencia-idD817-D480-FCA3
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0002-6329-0602
person.identifier.orcid0000-0002-1485-0423
person.identifier.orcid0000-0001-7916-9906
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0003-1705-2301
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridL-4327-2014
person.identifier.ridF-5696-2013
person.identifier.ridO-2638-2014
person.identifier.scopus-author-id7003730119
person.identifier.scopus-author-id55293251500
person.identifier.scopus-author-id54892791400
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id7202964185
person.identifier.scopus-author-id7102542478
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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