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ACE-inhibitory activity assay: IC50

2022-08-17Outro Acesso aberto
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dc.contributor.authorCoscueta, Ezequiel
dc.contributor.authorPintado, Maria Manuela
dc.date.accessioned2026-06-09T16:13:29Z
dc.date.available2026-06-09T16:13:29Z
dc.date.issued2022-08-17
dc.description.abstractThis protocol describes the procedure for the determination of the IC50 in inhibition on angiotensin-I converting enzyme (ACE) activity. ACE is also known as peptidyl dipeptidase A because it removes C-terminal dipeptides from a wide variety of peptide substrates. In the assay described here, the chosen substrate is the intramolecularly quenched fluorescent tripeptide o-aminobenzoylglycyl-p-nitrophenylalanylproline (Abz–Gly–Phe(NO2)–Pro). Hydrolysis of this substrate by the action of ACE generates the fluorescent product o-aminobenzoylglycine (Abz–Gly).eng
dc.identifier.doi10.17504/protocols.io.q26g74q5kgwz/v1
dc.identifier.other60d09b67-9ba4-4b2d-879e-e92b4a11f1a9
dc.identifier.urihttp://hdl.handle.net/10400.14/58056
dc.language.isoeng
dc.peerreviewedno
dc.publisherProtocols.io
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectACEeng
dc.subjectAngiotensin-converting enzymeeng
dc.subjectAntihypertensiveeng
dc.subjectIC50eng
dc.subjectBioactivityeng
dc.subjectAngiotensin-l converting enzymeeng
dc.subjectAngiotensineng
dc.subjectInhibitory activity assayeng
dc.subjectIc50 in inhibitioneng
dc.subjectNitrophenylalanylprolineeng
dc.subjectEnzymeeng
dc.subjectTerminal dipeptideeng
dc.subjectPeptide substrateeng
dc.subjectAction of aceeng
dc.subjectConverting enzymeeng
dc.subjectAssayeng
dc.titleACE-inhibitory activity assay: IC50
dc.typeother
dspace.entity.typePublication
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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