Repository logo
 
Publication

Genetic characterization of methicillin-resistant staphylococcus aureus isolates from human bloodstream infections: detection of mlsb resistance

2020Journal article Open access
Simple item page
dc.contributor.authorSilva, Vanessa
dc.contributor.authorHermenegildo, Sara
dc.contributor.authorFerreira, Catarina
dc.contributor.authorManaia, Celia M.
dc.contributor.authorCapita, Rosa
dc.contributor.authorAlonso-Calleja, Carlos
dc.contributor.authorCarvalho, Isabel
dc.contributor.authorPereira, José Eduardo
dc.contributor.authorMaltez, Luís
dc.contributor.authorCapelo, José L.
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorPoeta, Patricia
dc.date.accessioned2020-12-10T12:57:23Z
dc.date.available2020-12-10T12:57:23Z
dc.date.issued2020
dc.description.abstractIn this study we aimed to characterize antimicrobial resistance in methicillin-resistant Staphylococcus aureus (MRSA) isolated from bloodstream infections as well as the associated genetic lineages of the isolates. Sixteen MRSA isolates were recovered from bacteremia samples from inpatients between 2016 and 2019. The antimicrobial susceptibility of these isolates was tested by the Kirby–Bauer disk diffusion method against 14 antimicrobial agents. To determine the macrolide–lincosamide–streptogramin B (MLSB) resistance phenotype of the isolates, erythromycin-resistant isolates were assessed by double-disk diffusion (D-test). The resistance and virulence genes were screened by polymerase chain reaction (PCR). All isolates were characterized by multilocus sequence typing (MLST), spa typing, staphylococcal chromosomal cassette mec (SCCmec) typing, and accessory gene regulator (agr) typing. Isolates showed resistance to cefoxitin, penicillin, ciprofloxacin, erythromycin, fusidic acid, clindamycin, and aminoglycosides, confirmed by the presence of the blaZ, ermA, ermC, mphC, msrA/B, aac(6’)-Ie-aph(2’’)-Ia, and ant(4’)-Ia genes. Three isolates were Panton–Valentine-leukocidin-positive. Most strains (n = 12) presented an inducible MLSB phenotype. The isolates were ascribed to eight spa-types (t747, t002, t020, t1084, t008, t10682, t18526, and t1370) and four MLSTs (ST22, ST5, ST105, and ST8). Overall, most (n = 12) MRSA isolates had a multidrug-resistance profile with inducible MLSB phenotypes and belonged to epidemic MRSA clones.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, V., Hermenegildo, S., Ferreira, C., Manaia, C.M., Capita, R., Alonso-Calleja, C., …, Poeta, P. (2020). Genetic characterization of methicillin-resistant staphylococcus aureus isolates from human bloodstream infections: detection of mlsb resistance. Antibiotics, 9pt_PT
dc.identifier.doi10.3390/antibiotics9070375
dc.identifier.eid85090610273
dc.identifier.issn2079-6382
dc.identifier.pmcPMC7400445
dc.identifier.pmid32635147
dc.identifier.urihttp://hdl.handle.net/10400.14/31448
dc.identifier.wos000554180700001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationRTI2018-098267-R-C33pt_PT
dc.relationLE164G18pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMRSApt_PT
dc.subjectEMRSA-15pt_PT
dc.subjectMLSBpt_PT
dc.subjectBacteremiapt_PT
dc.subjectBloodstream infectionspt_PT
dc.titleGenetic characterization of methicillin-resistant staphylococcus aureus isolates from human bloodstream infections: detection of mlsb resistancept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume9pt_PT
person.familyNameSilva
person.familyNameManaia
person.familyNameCapita
person.familyNameAlonso-Calleja
person.familyNameMaltez
person.familyNameIgrejas
person.familyNamePoeta
person.givenNameVanessa
person.givenNameCélia
person.givenNameRosa
person.givenNameCarlos
person.givenNameLuís
person.givenNameGilberto
person.givenNamePatrícia
person.identifier591159
person.identifier.ciencia-id0610-A130-B5D2
person.identifier.ciencia-idC31F-553B-6365
person.identifier.ciencia-id6C13-274C-7EC3
person.identifier.ciencia-idD616-86BA-5EAF
person.identifier.orcid0000-0001-9406-8433
person.identifier.orcid0000-0002-3674-1789
person.identifier.orcid0000-0001-9584-0755
person.identifier.orcid0000-0002-8688-0912
person.identifier.orcid0000-0001-5896-4679
person.identifier.orcid0000-0002-6365-0735
person.identifier.orcid0000-0003-0925-689X
person.identifier.ridI-3671-2017
person.identifier.ridB-8192-2017
person.identifier.ridB-8189-2017
person.identifier.ridK-2924-2013
person.identifier.scopus-author-id57196704306
person.identifier.scopus-author-id6602465318
person.identifier.scopus-author-id55941342100
person.identifier.scopus-author-id7801595022
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione9139d7e-ba4f-4ea6-a71e-33180a3dcc54
relation.isAuthorOfPublicationa5f14577-0aed-4046-97da-5f4216c7aa07
relation.isAuthorOfPublication0316f33d-bd4a-48c7-b557-ddf8aa59f7c2
relation.isAuthorOfPublication6cff349f-1ae6-479d-902a-d5e1858d0b17
relation.isAuthorOfPublicationb623c80e-bdf3-4934-adf8-fe80fce6250e
relation.isAuthorOfPublication60f1d626-ee35-4bf1-83a3-d51294b00817
relation.isAuthorOfPublicatione7d6d956-da26-40dc-9626-86285f1b3511
relation.isAuthorOfPublication.latestForDiscovery0316f33d-bd4a-48c7-b557-ddf8aa59f7c2

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
20463898.pdf
Size:
255.37 KB
Format:
Adobe Portable Document Format
Download

Collections

CBQF - Contribuições em Revistas Científicas / Contribution to Journals