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Sequential injection system for the enzymatic determination of ethanol in wine

2006Journal article Restricted access
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dc.contributor.authorPáscoa, Ricardo N. M. J.
dc.contributor.authorVidigal, Susana S. M. P.
dc.contributor.authorTóth, Ildikó Varga
dc.contributor.authorRangel, António O. S. S.
dc.date.accessioned2011-10-22T11:01:54Z
dc.date.available2011-10-22T11:01:54Z
dc.date.issued2006
dc.description.abstractA sequential injection system was developed for the enzymatic determination of ethanol in wine. The spectrophotometric determination is based on the enzymatic reaction catalyzed by alcohol dehydrogenase in the presence of NAD+. The system was applied to the determination of ethanol in a range of 0.008-0.024% (v/v) with good repeatability; RSDn)10 < 2.3%. The results obtained with the developed system showed good agreement with those obtained by using the reference method. The determination rate was 25 h-1; 1 ímol of NAD+, 1.1 units of enzyme, and 50 íL of sample were consumed per determination; and the waste produced was 2.2 mL per assay.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citation"Journal of Agricultural and Food Chemistry" . ISSN 0021-8561. 54:1 (2006) 19-23por
dc.identifier.doi10.1021/jf051787l
dc.identifier.urihttp://hdl.handle.net/10400.14/6786
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Chemical Societypor
dc.subjectSequential injection analysispor
dc.subjectAlcohol dehydrogenasepor
dc.subjectEthanolpor
dc.subjectWinepor
dc.subjectSpectrophotometrypor
dc.titleSequential injection system for the enzymatic determination of ethanol in winepor
dc.typejournal article
dspace.entity.typePublication
person.familyNamePáscoa
person.familyNameVidigal
person.familyNameTóth
person.familyNameSantos Silva Rangel
person.givenNameRicardo
person.givenNameSusana
person.givenNameIldikó
person.givenNameAntónio Osmaro
person.identifier.ciencia-idBA14-0549-AA0A
person.identifier.ciencia-id2919-B77C-11C0
person.identifier.ciencia-idD61D-7CFB-178A
person.identifier.orcid0000-0003-2073-7643
person.identifier.orcid0000-0002-0213-063X
person.identifier.orcid0000-0002-7342-1063
person.identifier.orcid0000-0002-6486-8947
person.identifier.ridD-4791-2013
person.identifier.ridM-4661-2013
person.identifier.ridG-1733-2010
person.identifier.scopus-author-id11738933900
person.identifier.scopus-author-id11739488200
person.identifier.scopus-author-id35512332800
person.identifier.scopus-author-id7004827387
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication2140ed4c-4229-4ff5-9940-c7b476f09741
relation.isAuthorOfPublication59453cc2-ee50-4a1d-bbfc-924ca6417004
relation.isAuthorOfPublication7889563f-f3fc-4057-a97c-b51425b4dc46
relation.isAuthorOfPublication467be022-fd9c-4549-aa48-48ebcc1098eb
relation.isAuthorOfPublication.latestForDiscovery7889563f-f3fc-4057-a97c-b51425b4dc46

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