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Orientador(es)
Resumo(s)
A sequential injection system was developed for the enzymatic determination of ethanol in wine. The
spectrophotometric determination is based on the enzymatic reaction catalyzed by alcohol dehydrogenase
in the presence of NAD+. The system was applied to the determination of ethanol in a range
of 0.008-0.024% (v/v) with good repeatability; RSDn)10 < 2.3%. The results obtained with the
developed system showed good agreement with those obtained by using the reference method. The
determination rate was 25 h-1; 1 ímol of NAD+, 1.1 units of enzyme, and 50 íL of sample were
consumed per determination; and the waste produced was 2.2 mL per assay.
Descrição
Palavras-chave
Sequential injection analysis Alcohol dehydrogenase Ethanol Wine Spectrophotometry
Contexto Educativo
Citação
"Journal of Agricultural and Food Chemistry" . ISSN 0021-8561. 54:1 (2006) 19-23
Editora
American Chemical Society