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Biomimetic composite scaffold with phosphoserine signaling for bone tissue engineering application

2019-09-06Journal article Open access
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dc.contributor.authorSalgado, Christiane Laranjo
dc.contributor.authorTeixeira, Beatriz Isabel Brites
dc.contributor.authorMonteiro, Fernando Jorge Mendes
dc.date.accessioned2021-04-14T17:50:39Z
dc.date.available2021-04-14T17:50:39Z
dc.date.issued2019-09-06
dc.description.abstractIn guided bone tissue engineering, successful ingrowth of MSCs depends primarily on the nature of the scaffold. It is well-known that only seconds after implantation, biomaterials are coated by a layer of adsorbed proteins/peptides which modulates the subsequent cell/scaffold interactions, especially at early times after implantation. In this work, nanohydroxyapatite and collagen based composite materials (Coll/nanoHA) were modified with phosphorylated amino acid (O-phospho-L-serine–OPS) to mimic bone tissue, and induce cell differentiation. The choice for this phosphorylated amino acid is due to the fact that osteopontin is a serine-rich glycol-phosphoprotein and has been associated to the early stages of bone formation, and regeneration. Several concentrations of OPS were added to the Coll/nanoHA scaffold and physico-chemical, mechanical, and in vitro cell behavior were evaluated. Afterwards, the composite scaffold with stronger mechanical and best cellular behavior was tested in vivo, with or without previous in vitro culture of human MSC's (bone tissue engineering). The OPS signaling of the biocomposite scaffolds showed similar cellular adhesion and proliferation, but higher ALP enzyme activity (HBMSC). In vivo bone ectopic formation studies allowed for a thorough evaluation of the materials for MSC's osteogenic differentiation. The OPS-scaffolds results showed that the material could modulated mesenchymal cells behavior in favor of osteogenic differentiation into late osteoblasts that gave raised to their ECM with human bone proteins (osteopontin) and calcium deposits. Finally, OPS-modified scaffolds enhanced cell survival, engraftment, migration, and spatial distribution within the 3D matrix that could be used as a cell-loaded scaffold for tissue engineering applications and accelerate bone regeneration processes.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3389/fbioe.2019.00206pt_PT
dc.identifier.eid85072826869
dc.identifier.issn2296-4185
dc.identifier.pmcPMC6743420
dc.identifier.pmid31552233
dc.identifier.urihttp://hdl.handle.net/10400.14/32594
dc.identifier.wos000484808800001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiomaterialspt_PT
dc.subjectCollagenpt_PT
dc.subjectCryogel scaffoldpt_PT
dc.subjectGuided bone tissue regenerationpt_PT
dc.subjectNanohydroxyapatitept_PT
dc.subjectPhosphoserine modificationpt_PT
dc.titleBiomimetic composite scaffold with phosphoserine signaling for bone tissue engineering applicationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1360%2FCT0001/PT
oaire.citation.titleFrontiers in Bioengineering and Biotechnologypt_PT
oaire.citation.volume7pt_PT
oaire.fundingStreamDL 57/2016
person.familyNameSalgado
person.familyNameMonteiro
person.givenNameChristiane
person.givenNameFernando
person.identifier746127
person.identifier.ciencia-id2713-8B17-634C
person.identifier.ciencia-id5B1E-8842-AD62
person.identifier.orcid0000-0002-3844-1667
person.identifier.orcid0000-0002-1361-4605
person.identifier.ridK-5498-2013
person.identifier.scopus-author-id35327966600
person.identifier.scopus-author-id7006030702
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione55d98a4-c249-4258-b1bd-f399dddfb9b0
relation.isAuthorOfPublication7412d117-06a8-4dc4-b54a-f072d3002a25
relation.isAuthorOfPublication.latestForDiscoverye55d98a4-c249-4258-b1bd-f399dddfb9b0
relation.isProjectOfPublication48d6f1a5-1e16-4fa7-88d3-fcbec1a9b8db
relation.isProjectOfPublication.latestForDiscovery48d6f1a5-1e16-4fa7-88d3-fcbec1a9b8db

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