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Genotypic and phenotypic traits of blaCTX-M-carrying Escherichia coli strains from an UV-C-treated wastewater effluent

2020Journal article Open access
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dc.contributor.authorTavares, Rafael D. S.
dc.contributor.authorTacão, Marta
dc.contributor.authorFigueiredo, Ana S.
dc.contributor.authorDuarte, Ana S.
dc.contributor.authorEsposito, Fernanda
dc.contributor.authorLincopan, Nilton
dc.contributor.authorManaia, Célia M.
dc.contributor.authorHenriques, Isabel
dc.date.accessioned2020-08-07T10:47:05Z
dc.date.available2020-08-07T10:47:05Z
dc.date.issued2020
dc.description.abstractWastewater treatment plants (WWTPs) are relevant sources of antibiotic resistance into aquatic environments. Disinfection of WWTPs’ effluents (e.g. by UV-C irradiation) may attenuate this problem, though some clinically relevant bacteria have been shown to survive disinfection. In this study we characterized 25 CTX-M-producing Escherichia coli strains isolated from a WWTP’s UV-C-irradiated effluent, aiming to identify putative human health hazards associated with such effluents. Molecular typing indicated that the strains belong to the phylogroups A, B2 and C and clustered into 9 multilocus sequence types (STs), namely B2:ST131 (n ¼ 7), A:ST58 (n ¼ 1), A:ST155 (n ¼ 4), C:ST410 (n ¼ 2), A:ST453 (n ¼ 2), A:ST617 (n ¼ 2), A:ST744 (n ¼ 1), A:ST1284 (n ¼ 3) and a putative novel ST (n ¼ 3). PCR-screening identified 9 of the 20 antibiotic resistance genes investigated [i.e. sul1, sul2, sul3, tet(A), tet(B), blaOXA-1-like, aacA4, aacA4-cr and qnrS1]. The more prevalent were sul1, sul2 (n ¼ 15 isolates) and tet(A) (n ¼ 14 isolates). Plasmid restriction analysis indicated diverse plasmid content among strains (14 distinct profiles) and mating assays yielded cefotaxime-resistant transconjugants for 8 strains. Two of the transconjugants displayed a multi-drug resistance (MDR) phenotype. All strains were classified as cytotoxic to Vero cells (9 significantly more cytotoxic than the positive control) and 10 of 21 strains were invasive towards this cell line (including all B2:ST131 strains). The 10 strains tested against G. mellonella larvae exhibited a virulent behaviour. Twenty-four and 7 of the 25 strains produced siderophores and haemolysins, respectively. Approximately 66% of the strains formed biofilms. Genome analysis of 6 selected strains identified several virulence genes encoding toxins, siderophores, and colonizing, adhesion and invasion factors. Freshwater microcosms assays showed that after 28 days of incubation 3 out of 6 strains were still detected by cultivation and 4 strains by qPCR. Resistance phenotypes of these strains remained unaltered. Overall, we confirmed WWTP’s UV-C-treated outflow as a source of MDR and/or virulent E. coli strains, some probably capable of persisting in freshwater, and that carry conjugative antibiotic resistance plasmids. Hence, disinfected wastewater may still represent a risk for human health. More detailed evaluation of strains isolated from wastewater effluents is urgent, to design treatments that can mitigate the release of such bacteria.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationTavares, R. D., Tacão, M., Figueiredo, A. S., Duarte, A. S., Esposito, F., Lincopan, N., ... Henriques, I. (2020). Genotypic and phenotypic traits of blaCTX-M-carrying Escherichia coli strains from an UV-C-treated wastewater effluent. Water Research, 184, art. n.º 116079pt_PT
dc.identifier.doi10.1016/j.watres.2020.116079pt_PT
dc.identifier.eid85088365843
dc.identifier.issn0043-1354
dc.identifier.pmid32717492
dc.identifier.urihttp://hdl.handle.net/10400.14/30837
dc.identifier.wos000589969600006
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCEECIND/01304/2017pt_PT
dc.relationCEECINST/00137/2018pt_PT
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectWWTPpt_PT
dc.subjectAntibiotic resistancept_PT
dc.subjectVirulencept_PT
dc.subjectEnvironmental persistencept_PT
dc.subjectRiskpt_PT
dc.titleGenotypic and phenotypic traits of blaCTX-M-carrying Escherichia coli strains from an UV-C-treated wastewater effluentpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/WaterJPI%2F0002%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.citation.titleWater Researchpt_PT
oaire.citation.volume184pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameOliveira Martins Tacão
person.familyNameDuarte
person.familyNameLincopan
person.familyNameManaia
person.familyNameHenriques
person.givenNameMarta Cristina
person.givenNameAna Sofia
person.givenNameNilton
person.givenNameCélia
person.givenNameIsabel
person.identifierC-9785-2009
person.identifier295556
person.identifier.ciencia-idB118-1C8D-84A2
person.identifier.ciencia-id3813-8D79-4199
person.identifier.ciencia-idC31F-553B-6365
person.identifier.ciencia-idF516-BFA6-1588
person.identifier.orcid0000-0001-7965-4928
person.identifier.orcid0000-0001-6849-6004
person.identifier.orcid0000-0003-0161-5800
person.identifier.orcid0000-0002-3674-1789
person.identifier.orcid0000-0001-7717-4939
person.identifier.ridA-5212-2009
person.identifier.scopus-author-id8691053800
person.identifier.scopus-author-id56817825800
person.identifier.scopus-author-id6602465318
person.identifier.scopus-author-id6701586581
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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