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Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media

2020Journal article Restricted access
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dc.contributor.authorPintado, Ana I. E.
dc.contributor.authorFerreira, José A.
dc.contributor.authorPintado, Maria M. E.
dc.contributor.authorGomes, Ana M. P.
dc.contributor.authorMalcata, F. Xavier
dc.contributor.authorCoimbra, Manuel A.
dc.date.accessioned2019-12-09T19:10:30Z
dc.date.available2020-12-04T01:30:09Z
dc.date.issued2020
dc.description.abstractDe-Man Rogosa and Sharpe (MRS) is a complex medium commonly used to obtain exopolysaccharides (EPS) from lactic acid bacteria. However, the various nutrients (carbon and nitrogen sources) of media and supplements added to enhance the bacterial growth and EPS production, may interfere with the purification of the extracts resulting in an over-estimation of the EPS and erroneous structural assignments. The efficiency of trichloroacetic acid (TCA)/pronase and 5-sulfosalicylic acid – SSA methods was evaluated. In addition, the interference of the major MRS broth components as well as lactose was evaluated using xanthan gum as model control EPS. It was concluded that MRS medium is a major source of interfering compounds in the quantification of EPS, mainly glucose-rich material and to a lesser extent mannoproteins from yeast extract. This effect was found to be potentiated by the presence of lactose. TCA/pronase method was shown to be more efficient in eliminating interferents.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPintado, A. I., Ferreira, J. A., Pintado, M. M., Gomes, A. M., Malcata, F. X., Coimbra, M. A. (2020). Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media. Carbohydrate Polymers, 231, 115703.pt_PT
dc.identifier.doi10.1016/j.carbpol.2019.115703pt_PT
dc.identifier.eid85076256447
dc.identifier.issn0144-8617
dc.identifier.pmid31888825
dc.identifier.urihttp://hdl.handle.net/10400.14/28967
dc.identifier.wos000504650500022
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPOCTI/1999/AGR/36163pt_PT
dc.relationESTUDO DA PRODUÇÃO DE EXOPOLISSACARÍDEOS POR ESTIRPES PROBIÓTICAS
dc.relationTHE ROLE OF MITOCHONDRIA IN THE ENERGY METABOLISM OF TUMOR CELLS: A PROTEOME PERSPECTIVE
dc.subjectExopolysaccharidespt_PT
dc.subjectTrichloroacetic acid/pronasept_PT
dc.subject5-sulfosalicylic acidpt_PT
dc.subjectMRS brothpt_PT
dc.subjectYeast extractpt_PT
dc.subjectXanthan gumpt_PT
dc.subjectLactosept_PT
dc.titleEfficiency of purification methods on the recovery of exopolysaccharides from fermentation mediapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleESTUDO DA PRODUÇÃO DE EXOPOLISSACARÍDEOS POR ESTIRPES PROBIÓTICAS
oaire.awardTitleTHE ROLE OF MITOCHONDRIA IN THE ENERGY METABOLISM OF TUMOR CELLS: A PROTEOME PERSPECTIVE
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F5212%2F2001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F66288%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00062%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.citation.titleCarbohydrate Polymerspt_PT
oaire.citation.volume231
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNamePintado
person.familyNameFerreira
person.familyNamePintado
person.familyNameGomes
person.familyNameMalcata
person.familyNameCoimbra
person.givenNameAna
person.givenNameJosé Alexandre
person.givenNameMaria Manuela
person.givenNameAna Maria
person.givenNameFrancisco
person.givenNameManuel A.
person.identifier456608
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person.identifier.orcid0000-0001-7883-2446
person.identifier.orcid0000-0003-3073-1659
person.identifier.orcid0000-0001-8898-6342
person.identifier.ridF-5696-2013
person.identifier.ridB-9944-2013
person.identifier.ridL-2527-2014
person.identifier.scopus-author-id6602847645
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person.identifier.scopus-author-id57190668768
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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