Repository logo
 
Publication

Coimmobilization of L-asparaginase and glutamate dehydrogenase onto highly activated supports

2001Journal article Open access
Simple item page
dc.contributor.authorBalcão, Victor M.
dc.contributor.authorMateo, Cesar
dc.contributor.authorFernández-Lafuente, R.
dc.contributor.authorMalcata, F. Xavier
dc.contributor.authorGuisán, José M.
dc.date.accessioned2011-10-22T16:02:22Z
dc.date.available2011-10-22T16:02:22Z
dc.date.issued2001
dc.description.abstractIn the present research work, production of coimmobilized derivatives of L-asparaginase and glutamate dehydrogenase was attempted. Comparison of immobilization of each enzyme independently with coimmobilization of the two enzymes unfolded important advantages of the latter, namely a decrease in the induction period (time before the maximum reaction rate is virtually achieved) and an increase in the maximum reaction rate. The effectiveness of the independent enzyme derivatives was low; however, it was enhanced by three-fold when the enzymes were coimmobilized onto the same agarose-glutaraldehyde support. Each supporting agarose bead may in fact be viewed as a nano-reactor with in situ reaction and separation (i.e. elimination of the ammonia formed), with the nanoenvironment surrounding each enzyme molecule being essentially devoid of steric hindrance.por
dc.identifier.citationBALCÃO, Vitor M... [et al.] - Coimmobilization of L-asparaginase and glutamate dehydrogenase onto highly activated supports. Enzyme and Microbial Technology. ISSN 0141-0229. Vol. 28, n.º 7-8 (2001), p. 696–704por
dc.identifier.doi10.1016/S0141-0229(01)00307-6
dc.identifier.urihttp://hdl.handle.net/10400.14/6833
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectEnzymepor
dc.subjectAgarosepor
dc.subjectStructural stabilizationpor
dc.subjectImmobilizationpor
dc.subjectBiochemical engineeringpor
dc.subjectBiomedical engineeringpor
dc.titleCoimmobilization of L-asparaginase and glutamate dehydrogenase onto highly activated supportspor
dc.typejournal article
dspace.entity.typePublication
person.familyNameBalcão
person.familyNameMalcata
person.givenNameVictor
person.givenNameFrancisco
person.identifier.ciencia-idC314-A18C-931B
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0003-0772-2834
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridI-2182-2012
person.identifier.scopus-author-id6603449856
person.identifier.scopus-author-id7102542478
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication9b0860f7-99b1-4ded-a483-6435882272d0
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Coimmobilization of L-asparaginase and glutamate dehydrogenase onto.pdf
Size:
297.96 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.44 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ESB - Contribuições em Revistas Científicas / Contribution to Journals