Publication
PFAS biodegradation by Labrys portucalensis F11: evidence of chain shortening and identification of metabolites of PFOS, 6:2 FTS, and 5:3 FTCA
| dc.contributor.author | Wijayahena, Mindula K. | |
| dc.contributor.author | Moreira, Irina S. | |
| dc.contributor.author | Castro, Paula M. L. | |
| dc.contributor.author | Dowd, Sarah | |
| dc.contributor.author | Marciesky, Melissa I. | |
| dc.contributor.author | Ng, Carla | |
| dc.contributor.author | Aga, Diana S. | |
| dc.date.accessioned | 2025-04-04T14:49:07Z | |
| dc.date.available | 2025-04-04T14:49:07Z | |
| dc.date.issued | 2025-01-10 | |
| dc.description.abstract | The biodegradation of three per- and polyfluoroalkyl substances (PFAS), namely perfluorooctane sulfonic acid (PFOS), 6:2-fluorotelomer sulfonic acid (6:2 FTS), and 5:3-fluorotelomer carboxylic acid (5:3 FTCA), were evaluated using Labrys portucalensis F11, an aerobic bacteria known to defluorinate fluorine-containing compounds. Cultures of L. portucalensis F11 were grown in minimal salts media and treated with 10,000 μg/L of individual PFAS as the sole carbon source in separate flasks. In PFOS-spiked media, several metabolites were detected, including perfluoroheptane sulfonic acid (PFHpS), perfluorohexane sulfonic acid (PFHxS), perfluorohexanoic acid (PFHxA), perfluoropentanoic acid (PFPeA), perfluorobutanoic acid (PFBA), and perfluoropropanoic acid (PFPrA). After 194-day incubation three de-fluorinated metabolites were identified: PFOS-F (m/z = 480.940, PFOS-2F (m/z = 462.980), and unsaturated PFOS-3F (m/z = 442.943). During the biodegradation of 5:3 FTCA, the following metabolites were observed: PFHxA, PFPeA, PFBA, PFPrA, and two fluorotelomer unsaturated carboxylic acids (5:3 FTUCA and 7:2 FTUCA). The biodegradation of 6:2 FTS was slower, with only 21 % decrease in concentration observed after 100 days, and subsequent formation of 4:2 FTS. On the contrary, 90 % of PFOS and 58 % of 5:3 FTCA were degraded after 100 days. These results indicate that L. portucalensis F11 can be potentially used for PFAS biodegradation in contaminated environments. | eng |
| dc.identifier.doi | 10.1016/j.scitotenv.2024.178348 | |
| dc.identifier.eid | 85213870610 | |
| dc.identifier.issn | 0048-9697 | |
| dc.identifier.pmid | 39756302 | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/52928 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Per- and polyfluoroalkyl substances | |
| dc.subject | Aerobic biodegradation | |
| dc.subject | Defluorinated metabolites | |
| dc.subject | High-resolution mass spectrometry | |
| dc.subject | Ion mobility separation | |
| dc.title | PFAS biodegradation by Labrys portucalensis F11: evidence of chain shortening and identification of metabolites of PFOS, 6:2 FTS, and 5:3 FTCA | eng |
| dc.type | research article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 10 | |
| oaire.citation.startPage | 1 | |
| oaire.citation.title | Science of the Total Environment | |
| oaire.citation.volume | 959 | |
| oaire.version | http://purl.org/coar/version/c_ab4af688f83e57aa |